中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (2): 106-109.

• 论著 • 上一篇    下一篇

热休克蛋白10、60在皮肤鳞状细胞癌、基底细胞癌、日光性角化病中的表达

刘冬梅1,蒋亦秀2,张优拉3,黄池清1,付学峰4   

  1. 1. 浙江省金华市第五医院皮肤科
    2. 浙江金华市第五医院皮肤科
    3. 浙江省金华第五医院
    4. 浙江金华铁路医院皮肤科
  • 收稿日期:2010-08-04 修回日期:2010-09-19 出版日期:2011-02-15 发布日期:2011-02-10
  • 通讯作者: 刘冬梅 E-mail:ldm200370@126.com
  • 基金资助:

    浙江省金华市科技局

Expressions of heat shock protein （HSP） 60 and 10 in cutaneous squamous cell carcinoma, basal cell carcinoma and solar keratosis tissue

  • Received:2010-08-04 Revised:2010-09-19 Online:2011-02-15 Published:2011-02-10

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摘要：

目的 探讨热休克蛋白（HSP）10、60在皮肤鳞状细胞癌（SCC）、基底细胞癌（BCC）和日光性角化病（AK）中的表达水平。方法 采用免疫组化EnVision两步法测定HSP10、60在皮肤SCC、BCC、AK中的阳性表达水平，并与正常组对照。结果 与对照组比较，HSP10组只有BCC组的阳性表达高于正常组（Z = 3.24，P < 0.01），AK组（Z = 0.74，P > 0.05）和SCC组（Z = 0.52，P > 0.05）与对照组比较差异无统计学意义；HSP10组中AK与BCC，AK与SCC的差异有统计学意义（P < 0.05），但SCC与BCC组间差异无统计学意义（P > 0.05）。HSP60组三组的阳性表达均高于正常组，其中AK（Z = -2.90，P < 0.01）、BCC（Z = -2.15，P < 0.05）、SCC（Z = -2.78，P < 0.01）；三组间两两比较结果为AK = SCC > BCC（P < 0.05）。结论 HSP60的高表达可能与鳞状细胞癌、日光性角化病的生物行为有关。

关键词: 热休克蛋白

Abstract:

Objective To measure the expressions of HSP10 and 60 in cutaneous squamous cell carcinoma （SCC）, basal cell carcinoma （BCC） and solar keratosis （AK） tissue． Methods Lesion samples were resected from patients with SCC （n = 50）, BCC （n = 50） and AK （n = 50）, and control samples were obtained from the normal skin adjacent to the operation sites of 14 of the 50 patients with SCC, BCC and AK. Immunohistochemical Envision two step method was used to detect the expression of HSP60 and 10 in the tissue samples. Results The expression of HSP10 was significantly higher in BCC tissue samples （Z = 3.24, P < 0.001）, but not in AK （Z = 0.74, P > 0.05） or SCC （Z = 0.52, P > 0.05） tissue samples than in the normal control tissue samples. Statistical significance was observed in the expression of HSP10 between AK and SCC and between AK and BCC tissue samples （both P < 0.05）, but not between SCC and BCC tissue samples （P > 0.05）. Elevated expression of HSP60 was found in AK, BCC and SCC tissue samples compared with the control samples （Z = -2.90, -2.15, -2.78，P < 0.01, 0.05 and 0.01, respectively）. Furthermore, the expression of HSP60 in SCC tissue samples was higher than that in BCC tissue samples （P < 0.05） but similar to that in AK tissue samples. Conclusions There is likely to be a correlation between the high expression of HSP60 and biological behavior of SCC, and between the elevated HSP60 and HSP10 expressions and BCC initiation and development.

Key words: Heat shock rotein60