中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (12): 854-856.

• 论著 • 上一篇    下一篇

CXCR7在常见皮肤恶性肿瘤及其细胞株中的表达及意义

李小静1,刘排2,陈浩3,刘毅2,程芳4,5,张磊6,周武庆7,蒋明军3,孙建方3   

  1. 1. 河北邯郸 河北工程大学附属医院
    2. 中国医学科学院北京协和医学院皮肤病研究所
    3. 南京 中国医学科学院北京协和医学院皮肤病研究所
    4. 中国医学科学院皮肤病研究所
    5.
    6. 中国医学科学院中国协和医科大学皮肤病研究所
    7. 南京医科院皮研所
  • 收稿日期:2011-02-25 修回日期:2011-03-14 出版日期:2011-12-15 发布日期:2011-12-02
  • 通讯作者: 孙建方 E-mail:fangmin5758@yahoo.com.cn

Expression and significance of CXCR7 chemokine receptor in cutaneous malignant tumors and their cell lines

  • Received:2011-02-25 Revised:2011-03-14 Online:2011-12-15 Published:2011-12-02

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摘要：

目的 探讨趋化因子受体CXCR7在皮肤鳞状细胞癌、基底细胞癌、侵袭性皮肤黑素瘤及其细胞株中的表达及其意义。方法 收集30例皮肤鳞状细胞癌、25例基底细胞癌、30例皮肤黑素瘤的癌组织，采用免疫组织化学方法，检测CXCR7蛋白表达水平。采用RT-PCR、细胞免疫组化方法检测CXCR7在A375、M14、A431、HaCaT细胞株中mRNA及蛋白水平。结果 CXCR7在侵袭性皮肤黑素瘤中表达明显，高表达率为80%（24/30），皮肤鳞状细胞癌及基底细胞癌分别为26.67%（8/30）、8%（2/25）；皮肤黑素瘤CXCR7高表达率与鳞状细胞癌、基底细胞癌比较，差异均有统计学意义（χ2值分别为17.16和28.36，P值均 < 0.05）。CXCR7 mRNA在A375、M14、A431细胞株中均可检出，其中A375表达最强，而HaCaT细胞不表达；细胞免疫组化显示，仅在A375细胞见棕黄色颗粒着色。结论 皮肤黑素瘤及其细胞株A375高表达CXCR7，其可能参与了其恶性侵袭与转移。

关键词: 细胞系，肿瘤

Abstract:

Objective To investigate the expression of CXCR7 in several cutaneous malignant tumors including cutaneous squamous cell carcinoma （SCC）, basal cell carcinoma （BCC） and invasive cutaneous malignant melanoma and their cell lines, as well as its significance. Methods Tissue specimens were obtained from the lesions of 30 patients with cutaneous squamous cell carcinoma, 25 patients with basal cell carcinoma and 30 patients with cutaneous malignant melanoma. Immunohistochemistry was performed to detect the expression of CXCR7 protein in these tissue specimens and several cell lines （A375 human melanoma cells, M14 human melanoma cells, A431 human epidermoid carcinoma cells, HaCaT human keratinocytes）. The mRNA expression of CXCR7 in these cell lines was measured by reverse transcription PCR. Results CXCR7 protein was apparently expressed in invasive cutaneous malignant melanoma. The high expression rate of CXCR7 protein was significantly elevated in cutaneous malignant melanoma tissue specimens compared with SCC and BCC tissue specimens 〔80% （24/30） vs. 26.67% （8/30） and 8% （2/25）, ?字2 = 17.16, 28.36, both P < 0.05〕. CXCR7 mRNA was expressed in A375, M14 and A431 cells, but not in HaCaT cells, with the strongest expression observed in A375 cells. Immunohistochemistry revealed the expression of CXCR7 protein only in A375 cells. Conclusions CXCR7 is highly expressed in cutaneous malignant melanoma and A375 cells,which may be involved in the malignant invasion and metastasis of melanoma.