中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (11): 805-807.

• 研究报道 • 上一篇    下一篇

人疱疹病毒8型ORF26单核苷酸多态性研究

吴秀娟1,石岩1,靳颖1,张德志2,普雄明3   

  1. 1. 新疆维吾尔自治区人民医院皮肤性病科
    2. 新疆维吾尔自治区人民医院
    3. 乌鲁木齐市新疆维吾尔自治区人民医院皮肤科
  • 收稿日期:2011-01-10 修回日期:2011-03-03 出版日期:2011-11-15 发布日期:2011-11-01
  • 通讯作者: 普雄明 E-mail:puxiongming3@sina.com

Single nucleotide polymorphisms in the open reading frame 26 （ORF26） gene of human herpesvirus 8 （HHV-8） in Kaposi′s sarcoma

  • Received:2011-01-10 Revised:2011-03-03 Online:2011-11-15 Published:2011-11-01

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摘要：

目的 探讨人疱疹病毒8型（HHV-8）ORF26的单核苷酸多态性，分析其与Kaposi肉瘤不同临床分型及黏膜侵袭性的相关性。方法 Kaposi肉瘤患者32例，其中经典型26例，艾滋病相关型6例。使用酚-氯仿-异戊醇方法对Kaposi肉瘤石蜡包埋组织进行HHV-8 DNA抽提，采用巢式PCR方法扩增ORF26基因并双向测序，使用DNAStar软件和Clustal W软件分析ORF26基因的单核苷酸多态性。运用Fisher确切概率法对结果进行统计学分析。结果 ORF26基因研究发现，32例Kaposi肉瘤患者中HHV-8阳性30例，6例艾滋病相关型HHV-8均为阳性。30例患者的病毒株中，HHV-8 ORF26基因SNP主要集中在981T/C（12例）、1086C/T（12例）、1139A/C（12例）；HHV-8 ORF26基因单核苷酸多态性在不同临床分型或有无黏膜损害的Kaposi肉瘤之间的差异无统计学意义。结论 HHV-8 ORF26基因单核苷酸多态性可能与Kaposi肉瘤不同临床分型和黏膜侵袭性无关。

关键词: 单核苷酸多态性

Abstract:

Objective To study the single nucleotide polymorphisms （SNPs） in the ORF26 gene of HHV-8 in Kaposi′s sarcoma （KS）, and to assess their correlations with the clinical phenotype and mucosal invasion of KS. Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from paraffin-embedded tissue specimens obtained from 32 cases of KS （including 26 classic and 6 AIDS-related KS）. The ORF26 gene of HHV-8 was amplified by nested-PCR followed by bidirectional sequencing. The software DNAStar and program Clustal W were used to assess the SNPs in the ORF26 gene. Statistical analysis was carried out by using the Fisher′s exact probability test. Results HHV-8 DNA was detected in 30 of the 32 tissue specimens, and in all of the 6 AIDS-related specimens. The predominant SNPs were 981 T/C （n = 12）, 1086 C/T （n = 12） and 1139 A/C （n = 12） in the ORF26 gene of the 30 strains of HHV-8. No significant difference was observed in the distribution of SNPs in ORF26 between different phenotypes of KS or between KS with and without mucosal invasion. Conclusion The ORF26 SNPs of HHV-8 seem unrelated to the clinical phenotypes or mucosal invasion of KS.

Key words: Single nucleotide polymorphisms