中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (11): 797-799.

• 论著 • 上一篇    下一篇

强脉冲光对巴马香猪糖皮质激素诱导的皮炎模型皮肤组织病理的影响

王国江1,董达科2,陈向明3,张海清1,顾军4   

  1. 1. 上海嘉定区南翔医院皮肤科
    2. 上海市嘉定区南翔医院皮肤科
    3. 上海市嘉定区南翔医院
    4. 上海第二军医大学附属长海医院皮肤科
  • 收稿日期:2011-01-13 修回日期:2011-07-14 出版日期:2011-11-15 发布日期:2011-11-01
  • 通讯作者: 顾军 E-mail:gujun79@163.com
  • 基金资助:

    上海市嘉定区卫生局科研基金项目

Dermatopathological changes in a Bama miniature pig model of steroid-induced dermatitis after treatment with intense pulsed light

  • Received:2011-01-13 Revised:2011-07-14 Online:2011-11-15 Published:2011-11-01
  • Contact: GU Jun E-mail:gujun79@163.com

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摘要：

目的 探讨强脉冲光对巴马香猪糖皮质激素诱导的皮炎模型皮肤组织病理的影响。方法 选用2月龄雌性巴马香猪5只，将颈部两侧白色皮毛区作为实验靶皮肤区域，右侧外用0.05%卤米松乳膏每日涂药2次，连续用药60 d，形成糖皮质激素诱导的皮炎模型。随机选3只给予强脉冲光治疗糖皮质激素诱导的皮炎区域，能量为25 J/cm2，每3周1次，共3次；另2只不给予治疗作为自然恢复组观察9周。局部皮肤组织做HE染色，观察各组皮肤病理变化。结果 巴马香猪糖皮质激素诱导的皮炎模型组经强脉冲光治疗后，表皮角质形成细胞层数为（6.27 ± 1.26）层，较自然恢复组（2.98 ± 0.92层）明显增加（t = 3.27，P ＜ 0.01）；真皮胶原纤维厚度为（1.88 ± 0.19） mm，也较自然恢复组（0.84 ± 0.15 mm）明显增加（t = 4.25，P ＜ 0.01），真皮内扩张的毛细血管消退。结论 强脉冲光能有效增加皮肤厚度，消除皮肤红斑，增加皮肤弹性。

关键词: 皮肤病理

Abstract:

Objective To assess the impact of intense pulsed light （IPL） on the dermatopathological manifestation in a Bama miniature pig model of steroid-induced dermatitis. Methods Five female Bama miniature pigs aged two months were selected. The white skin areas with white hair at both sides of the neck served as the target area. Halometasone （0.05%） cream was applied to the right target area twice daily for 60 days to establish a model of steroid-induced dermatitis. Then, 3 pigs were randomly selected and irradiated with IPL of 25 J/cm2 at the model area with an interval of 3 weeks for 9 weeks, the remaining 2 pigs receiving no treatment served as the natural recovery group. Finally, skin tissues were obtained from the left and right target areas and subjected to haematoxylin and eosin staining for the observation of histopathological changes. Results A significant increase was observed in the layer number of keratinocytes and thickness of dermal collagen fiber in the IPL-treated pigs compared with the pigs in natural recovery group（6.27 ± 1.26 vs. 2.98 ± 0.92, t = 3.27, P ＜ 0.01; 1.88 ± 0.19 mm vs. 0.84 ± 0.15 mm, t = 4.25, P ＜ 0.01）. Moreover, IPL irradiation resulted in the regression of telangiectasis in the dermis. Conclusion IPL may increase skin thickness, relieve flushing and improve skin elasticity efficiently.

Key words: dermatopathology