中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (11): 761-764.

• 论著 •    下一篇

芳香烃受体在人表皮、毛囊和皮脂腺上的表达及其意义

鞠强1,余茜2,宋宁静2,谈亦2,夏隆庆3,Christos C. Zouboulis4   

  1. 1. 上海市皮肤病性病医院
    2. 上海市皮肤病医院
    3. 南京 中国医学科学院北京协和医学院皮肤病研究所
    4. Dessau Medical Center
  • 收稿日期:2011-02-24 修回日期:2011-04-29 出版日期:2011-11-15 发布日期:2011-11-01
  • 通讯作者: 鞠强 E-mail:qiangju@yahoo.com.cn
  • 基金资助:

    国家自然科学基金;上海市自然科学基金

Expression of aryl hydrocarbon receptor in human epidermis, hair follicles and sebaceous glands and its significance

  • Received:2011-02-24 Revised:2011-04-29 Online:2011-11-15 Published:2011-11-01
  • Contact: qiang ju E-mail:qiangju@yahoo.com.cn

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摘要：

目的 观察芳香烃受体（AhR）在人表皮、毛囊及皮脂腺细胞上的表达及在二噁英作用下的激活情况。方法 采用免疫荧光和免疫组化法观察AhR蛋白在人面部表皮、毛囊、皮脂腺及体外培养的HaCaT细胞和SZ95人皮脂腺细胞上的表达情况；Western印记法观察2,3,7,8-四氯二苯-p-二噁英（TCDD）作用HaCaT细胞和SZ95细胞3 d后AhR蛋白表达的变化情况。结果 AhR蛋白在人面部皮肤表皮、毛囊、皮脂腺细胞上表达，并且AhR蛋白在表皮棘层和颗粒层的表达强于基底细胞层，在皮脂腺小叶的外周和中央表达强于皮脂腺小叶中间，在毛囊外毛根鞘和近毛小皮细胞上的表达强于内毛根鞘细胞。AhR蛋白在HaCaT细胞和SZ95细胞的细胞核和胞质中高表达。在TCDD作用下，HaCaT细胞和SZ95细胞内AhR蛋白表达明显下降，显示AhR在TCDD作用下被激活。结论 AhR在人表皮、毛囊、皮脂腺、HaCaT细胞及SZ95细胞上高表达，并在TCDD作用下激活，显示二噁英有可能通过AhR信号途径引起了人表皮、毛囊和皮脂腺的异常分化。

关键词: HaCat细胞：SZ95细胞

Abstract:

Objective To observe the expression of aryl hydrocarbon receptor （AhR） in human epidermis, hair follicles and sebaceous glands and the activation of AhR by dioxin. Methods Full-thickness skin specimens were obtained from 3 volunteers receiving cosmetic facial surgery. Indirect immunofluorescence and immunohistochemistry were used to detect the expression of AhR in these skin specimens, as well as on cultured human HaCaT keratinocytes and SZ95 sebocytes. Western blot was performed to detect the expressions of AhR protein on HaCaT cells and SZ95 sebocytes after 3-day treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD）. Results AhR protein was found to be expressed in human epidermis, hair follicles and sebaceous glands, and the expression was stronger in the stratum spinosum and stratum granulosum than in the basal layer, in the outer and central layer than in the middle layer of sebaceous lobuli, in hair follicle outer root sheath and hair cuticles than in the inner root sheath. There was a high expression of AhR in the nucleus and cytoplasm of HaCaT and SZ95 cells. A significant decrease was observed in the expression of AhR in both HaCaT cells and SZ95 cells after treatment with TCDD for 3 days, hinting that AhR was activated by TCDD. Conclusions AhR is highly expressed in human epidermis, hair follicles and sebaceous glands, and can be activated by TCDD, suggesting that dioxin may induce the abnormal differentiation of epidermis, hair follicles and sebaceous glands via AhR singaling pathway.

Key words: HaCat keratinocytes：SZ95 sebocytes