中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (10): 704-707.

• 论著 • 上一篇    下一篇

微量液基稀释法与琼脂稀释法测定马拉色菌体外抗真菌药物敏感性

徐圣经1,曹双林2,夏继宁3,沈永年4,吕桂霞4,刘维达4,傅琳玲5,丁琦2   

  1. 1. 镇江市第一人民医院
    2. 南通大学附属医院
    3. 浙江省嘉兴市中医医院
    4. 南京 中国医学科学院北京协和医学院皮肤病研究所
    5. 南通大学附属医院皮肤科
  • 收稿日期:2010-11-15 修回日期:2011-05-24 出版日期:2011-10-15 发布日期:2011-09-30
  • 通讯作者: 曹双林 E-mail:slcao1@hotmail.com
  • 基金资助:

    南通市科技局社会发展项目(S2006023)

Comparison of broth microdilution and agar dilution methods for antifungal susceptibility testing of Malassezia species in vitro

  • Received:2010-11-15 Revised:2011-05-24 Online:2011-10-15 Published:2011-09-30
  • Contact: shuanglin Cao E-mail:slcao1@hotmail.com

摘要:

目的 比较微量液基稀释法与琼脂稀释法检测马拉色菌体外对氟康唑、酮康唑及伊曲康唑敏感性的差异。方法 对临床分离的5种27株马拉色菌,分别用微量液基稀释法与琼脂稀释法测定氟康唑、酮康唑及伊曲康唑对这些马拉色菌的最低抑菌浓度(MIC)。结果 微量液基稀释法显示氟康唑MIC值范围0.25 ~ ≥ 64 mg/L,酮康唑≤0.03 ~ 0.5 mg/L,伊曲康唑≤0.03 ~ 0.125 mg/L;琼脂稀释法显示氟康唑MIC值范围2 ~ ≥ 64 mg/L,酮康唑≤0.03 ~ 0.5 mg/L,伊曲康唑≤0.03 ~ 0.25 mg/L。两种方法测定3种唑类抗真菌药对马拉色菌的活性由高到低为:伊曲康唑、酮康唑、氟康唑。两种方法测得氟康唑、酮康唑及伊曲康唑MIC值符合率分别为78.8%、85.2%、88.9%,组内相关系数分别为0.88、0.80、0.76。结论 氟康唑、酮康唑及伊曲康唑对马拉色菌均有较好的抗菌活性,以伊曲康唑对各菌种的抗菌活性最强。微量液基稀释法与琼脂稀释法相比具有良好的一致性,均适用于马拉色菌体外药敏试验。

关键词: 最低抑菌浓度

Abstract:

Objective To compare broth microdilution and agar dilution methods for in vitro testing of activities of fluconazole, ketoconazole and itraconazole against clinical Malassezia isolates. Methods Broth microdilution and agar dilution methods were used to determine the minimal inhibitory concentration (MIC) of fluconazole, ketoconazole and itraconazole for 27 clinical strains (5 species) of Malassezia. Results The minimal inhibitory concentration (MIC) ranges of fluconazole, ketoconazole and itraconazole were 0.25 - ≥64 mg/L, ≤0.03 - 0.5 mg/L and ≤0.03 - 0.125 mg/L respectively as shown by broth microdilution method, 2 - ≥64 mg/L, ≤0.03 - 0.5 mg/L and ≤0.03 - 0.25 mg/L respectively as revealed by agar dilution method. Both methods demonstrated that itraconazole possessed the strongest activity against Malassezia species, followed by ketoconazole and fluconazole. The agreement rate in MICs between the two methods was 78.8%, 85.2% and 88.9%, respectively for fluconazole, ketoconazole and itraconazole, with the intraclass correlation coefficients (ICCs) being 0.88, 0.80 and 0.76 respectively. Conclusions Fluconazole, ketoconazole and itraconazole are highly active against Malassezia species in vitro, and itraconazole is the most active. Broth microdilution and agar dilution method coincide well in, and are applicable for, the antifungal susceptibility testing of Malassezia species in vitro.

Key words: MIC

中图分类号: 

  • R756.9