中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (1): 35-38.

• 论著 • 上一篇    下一篇

IFN-γ联合全反式维A酸对人皮肤鳞状细胞癌SCL-1细胞生长凋亡的影响

王珍1,刘梅2,李铁男3,朱红4,王雅坤4,何春涤5   

  1. 1. 沈阳市第七人民医院；中国医科大学附属第一医院
    2. 沈阳市中国医科大学附属第一医院皮肤科
    3. 沈阳市第七人民医院
    4. 沈阳 中国医科大学附属第一医院皮肤科
    5. 中国医科大学附属第一医院皮肤科
  • 收稿日期:2010-04-23 修回日期:2010-08-08 出版日期:2011-01-15 发布日期:2011-01-10
  • 通讯作者: 何春涤 E-mail:chundihe@hotmail.com
  • 基金资助:

    教育部高等学校博士学科点专项科研基金;辽宁省教育厅创新团队项目计划;沈阳市卫生局科研课题计划项目

Effect of interferon-γ and all-trans retinoic acid （ATRA） on the proliferation of and apoptosis in a human cutaneous squamous cell carcinoma cell line SCL-1

  • Received:2010-04-23 Revised:2010-08-08 Online:2011-01-15 Published:2011-01-10
  • Contact: HE Chun-di E-mail:chundihe@hotmail.com

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摘要：

目的 探讨IFN-γ联合全反式维A酸（ATRA）在体外对人皮肤鳞状细胞癌SCL-1细胞形态分化、增殖及生长凋亡的影响。方法 SCL-1细胞根据处理因素不同分为，①对照组；②1 μmol/L ATRA组；③100 U/ml IFN-γ组；④500 U/ml IFN-γ组；⑤1000 U/ml IFN-γ组；⑥1000 U/ml IFN-γ + 1 μmol/L ATRA组。用噻唑蓝（MTT）和流式细胞仪（FCM）分析细胞增殖及凋亡的变化。透射电镜、1%碘化丙啶染色及倒置相差显微镜观察细胞凋亡形态。结果 细胞的增殖抑制有明显IFN-γ浓度依赖性，其中1000 U/ml浓度抑制作用最明显。ATRA及IFN-γ能诱导SCL-1细胞凋亡，凋亡率分别是4.84%和11.96%，二者联合诱导凋亡率为18.71%；透射电镜及1%碘化丙啶染色可见1 μmol/L ATRA和1000 U/ml IFN-γ处理的SCL-1细胞发生一系列凋亡特征性的形态学改变，并见较多早期凋亡细胞。IFN-γ及ATRA诱导SCL-1细胞形态呈双极性改变，趋向良性分化。结论 IFN-γ可促进SCL-1细胞分化，抑制其增殖，在一定范围内有剂量依赖性，且与ATRA具有协同作用。

关键词: 电镜

Abstract:

Objective To investigate the in vitro effect of interferon-γ （IFN-γ） and ATRA on the morphological transition, proliferation of and apoptosis in a human cutaneous squamous cell carcinoma cell line SCL-1. Methods Cultured SCL-1 cells were divided into 6 groups to be treated with ATRA of 1 μmol/L, various concentrations （100, 500, 1000 U/ml） of IFN-γ, the combination of ATRA of 1 μmol/L and IFN-γ of 1000 U/ml, respectively, or to remain untreated. MTT assay and flow cytometry were performed to evaluate the cell proliferation and apoptosis. The morphological features of apoptotic cells were observed by a transmission electron microscope （TEM） and inverted phase contrast microscope after 1% propidium iodide staining. Results IFN-γ could inhibit the proliferation of SCL-1 cells in a dose-dependent manner, and the most pronounced inhibitory effect was observed at a dose of 1000 U/ml . ATRA and IFN-γ induced an apoptosis in SCL-1 cells, and the early apoptosis rate was 4.84%, 11.96% and 18.71% in SCL-1 cells after treated with ATRA of 1 μmol/L, IFN-γ of 1000 U/ml and their combination, respectively. A series of morphological changes characteristic of apoptosis, such as bipolar changes, were observed in SCL-1 cells treated with ATRA and IFN-γ, with the presence of many early apoptotic cells, which showed a trend towards benign differentiation. Conclusions Within a certain concentration range, IFN-γ can promote the differentiation, but inhibit the proliferation of SCL-1 cells in a dose-dependent manner, and ATRA could enhance the effects of IFN-γ.

Key words: TEM