中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (8): 546-548.

• 论著 • 上一篇    下一篇

断发毛癣菌致黑点癣一例及菌体胞外酶活性分析

张瑞峰1,冉玉平2,代亚玲3   

  1. 1. 四川大学华西医院皮肤科
    2. 成都市四川大学华西医院皮肤性病科
    3.
  • 收稿日期:2010-02-01 修回日期:2010-02-14 出版日期:2010-08-15 发布日期:2010-08-10
  • 通讯作者: 张瑞峰 E-mail:zrfsxhz@126.com
  • 基金资助:

    教育部高等学校博士学科点专项基金

Black-dot ringworm caused by Trichophyton tonsurans and analysis of its extracellular enzymatic activity

  • Received:2010-02-01 Revised:2010-02-14 Online:2010-08-15 Published:2010-08-10
  • Contact: zhang ruifeng E-mail:zrfsxhz@126.com

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摘要：

目的 报道1例发生在3岁女孩的由断发毛癣菌所致黑点癣。方法 取病发标本作真菌直接镜检和培养，对培养菌株进行形态学鉴定、生化鉴定、分子生物学鉴定。分析本菌株胞外酶活性。结果 KOH涂片可见发干内充满孢子，典型菌落呈灰白绒毛样质地，小培养见蜈蚣样侧生棒状小分生孢子，尿素酶实验阳性。扩增真菌rDNA的ITS区得到687 bp的片段，测序后比对与基因库中多株断发毛癣菌同源性100%。胞外酶活性分析见碱性磷酸酶、酸性磷酸酶、酯酶、β-葡萄糖苷酶、白氨酸芳胺酶、N-乙酰-葡萄糖胺酶、α-甘露糖苷酶活性较高。结论 根据真菌形态学特征、生化特性及DNA序列分析，鉴定本致病菌株为断发毛癣菌。诊断为黑点癣。

关键词: 黑点癣, 断发毛癣菌, 胞外酶活性

Abstract:

Objective　To report a case of black-dot ringworm caused by Trichophyton tonsurans in a 3-year-old girl. Methods　Lesional hair was obtained from the patient and subjected to direct microscopic examination as well as culture. Subsequently, the isolate underwent morphological, biochemical and molecular biology identification. The extracellular enzymatic activity of the isolate was analyzed. Results　Microscopy revealed that the hair shaft was filled with fungal spores. Typical colony of the isolate was grayish-white with downy appearance. Slide culture showed centipede-like, lateral, rod-shaped microconidia. Urease test was positive. The amplification of ribosomal DNA （rDNA） ITS domains by PCR produced a 687 bp-sized fragment which had a 100% homology with the sequences of several Trichophyton tonsurans strains in the GenBank database. The extracellular enzymatic activity analysis showed an increase in the activity of alkaline phosphatase, acid phosphatase, esterase （C4）, β-glucosidase, leucine arylamidase, N-acetyl-β-glucosaminidase and α-mannosidase. Conclusions　The pathogenic fungus is identified as Trichophyton tonsurans based on morphological and biochemical features as well as sequence of the ITS region of rDNA, and the child was diagnosed with black-dot ringworm.

Key words: Black-dot ringworm, Trichophyton tonsurans, extracellular enzymatic activity