中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (7): 493-496.

• 论著 • 上一篇    下一篇

姜黄素对THP-1细胞Toll样受体4信号传导通路中相关因子mRNA水平的影响

彭立新1,李新宇2,宋莎莎2,王永芳2,徐兰芳3   

  1. 1. 中国医学科学院北京协和医学院皮肤病研究所药研室
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
    3. 南京医科院皮研所
  • 收稿日期:2009-10-30 修回日期:2010-03-09 出版日期:2010-07-15 发布日期:2010-07-13
  • 通讯作者: 彭立新 E-mail:peng.li.xin@hotmail.com

Effects of curcumin on mRNA expression of cytokines related to Toll-like receptor 4 signaling in THP-1 cells

  • Received:2009-10-30 Revised:2010-03-09 Online:2010-07-15 Published:2010-07-13

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摘要：

目的 研究姜黄素对人单核细胞株THP-1 Toll样受体4（TLR4）信号传导通路中相关因子mRNA水平的影响。方法 用不同浓度姜黄素（50、25、12.5 mg/L）预处理THP-1细胞12 h，0.043 mg/L地塞米松处理组作为阳性对照，继而用1 mg/L脂多糖（LPS）刺激诱导细胞4 h，以不做处理的THP-1作为阴性对照，仅用1 mg/L LPS刺激的THP-1作为LPS刺激组。提取细胞总RNA，用RT-PCR方法检测TLR4信号传导通路中TNF受体相关因子（TRAF）6、IL-1受体相关激酶（IRAK1）、核因子κB（NF-κB）mRNA的表达情况。结果 未经处理的THP-1细胞经LPS刺激4 h，可以显著提高TRAF6、IRAK1、NF-κB mRNA的表达，与阴性对照组比较，t值分别为38.69、39.13、23.99（P ＜ 0.01）。50、25 mg/L姜黄素可以明显抑制THP-1细胞经LPS诱导后TRAF6、IRAK1、NF-κB mRNA的表达（P值均 ＜ 0.01），抑制率均在50%以上。结论 一定浓度的姜黄素可以抑制LPS诱导后THP-1细胞TRAF6、IRAK1、NF-κB mRNA的表达，表明姜黄素对TLR4信号传导通路中相关因子mRNA水平有调节作用。

关键词: 姜黄素, TNF受体相关因子6, 白细胞介素1受体相关激酶类, NF-κB, 细胞系

Abstract:

Objective To investigate the effects of curcumin on mRNA expression of cytokines related to Toll-like receptor （TLR） 4 signaling in THP-1 cells. Methods After pretreatment with different concentrations （50, 25, 12.5 mg/L） of curcumin or dexamethasone for 12 hours, THP-1 cells were stimulated by lipopolysaccharide （LPS, 1 mg/L） for 4 hours followed by the collection of cells. Then total RNA was isolated from these cells and subjected to reverse transcription-polymerase chain reaction （RT-PCR） for the detection of mRNA expression of tumor necrosis factor receptor-associated factor 6 （TRAF6）, interleukin-1 receptor-associated kinase （IRAK1） and nuclear factor （NF）-κB. THP-1 cells without pretreatment or stimulation served as negative control, and those only stimulated with LPS served as LPS group. Results After stimulation with LPS （1 mg/L） for 4 hours, the mRNA expressions of TRAF6, IRAK1 and NF-κB were significantly upregulated in THP-1 cells compared with negative control cells （t = 38.69, 39.13, 23.99, all P < 0.01）. Curcumin of 50 mg/L and 25 mg/L significantly inhibited the mRNA expressions of TRAF6, IRAK1 and NF-κB upregulated by LPS with an inhibition rate of more than 50% （all P < 0.01）. Conclusions Certain concentrations of curcumin can inhibit the mRNA expressions of TRAF6, IRAK1 and NF-κB，which demonstrates the regulatory effect of curcumin on the mRNA expressions of TLR4 signaling pathway-associated cytokines.

Key words: Curcumin, TNF receptor-associated factor 6, Interleukin-1 receptor-associated kinases, NF-kappa B, Cell line