中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (7): 482-484.

• 论著 • 上一篇    下一篇

沙眼衣原体泌尿生殖道感染患者血清Pmp蛋白抗体的检测

李燕1,刘原君2,齐蔓莉1,盛彩虹3,刘全忠1   

  1. 1. 天津医科大学总医院皮肤性病科
    2. 天津医科大学总医院/天津性传播疾病研究所
    3. 天津医科大学总医院
  • 收稿日期:2009-11-10 修回日期:2010-03-23 出版日期:2010-07-15 发布日期:2010-07-13
  • 通讯作者: 李燕 E-mail:liliang0831@sina.com
  • 基金资助:

    国家自然科学基金

Detection of serum antibodies to polymorphic membrane proteins （Pmp） in patients with urogenital Chlamydia trachomatis infection

  • Received:2009-11-10 Revised:2010-03-23 Online:2010-07-15 Published:2010-07-13

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摘要：

目的 检测沙眼衣原体（C.t）泌尿生殖道感染患者多形外膜蛋白（Pmp）的血清抗体，初步探讨Pmp蛋白与C.t泌尿生殖道感染的相关性。方法 从临床选择20例正常人作为阴性对照组，77例C.t泌尿生殖道感染患者作为阳性对照组，随访43例患者3个月，分为C.t持续感染组（19例）与转阴组（24例），采集患者和对照组的血清，Western印迹法检测Pmp蛋白血清抗体（Pmp-Ab），分组比较Pmp-Ab的阳性率，并同临床资料进行相关性分析。结果 Pmp-Ab阳性率在C.t感染组为90.20%（71/77），阴性对照组为20%（4/20），感染组明显高于阴性对照组（P < 0.05）。在C.t感染组，9种Pmp-Ab都可检测到，但不同抗体的阳性率不同，PmpA ～ PmpI分别为61.04%（47例）、88.31%（68例）、63.63%（49例）、28.57%（22例）、63.63%（49例）、75.32%（58例）、62.34%（48例）、77.92%（60例）和70.13%（54例）；其中PmpB-Ab阳性率最高， PmpD-Ab阳性率最低。持续感染组与转阴组9种Pmp-Ab阳性率差异均无统计学意义。结论 C.t泌尿生殖道感染患者可产生针对Pmp蛋白的抗体，不同的Pmp蛋白免疫原性有差别，Pmp蛋白抗体对机体的免疫保护作用不强，血清抗体存在个体差异。E型C.t 的9种Pmp蛋白全部表达。

关键词: 沙眼衣原体, 多形外膜蛋白, 抗体检测, 免疫原性

Abstract:

Objective To detect serum antibodies to Pmp in patients with urogenital Chlamydia trachomatis infection and to assess the relationship between Pmp and urogenital C. trachomatis infection. Methods Twenty healthy adults and 77 patients with urogenital C. trachomatis infection were recruited into this study. A 3-month follow-up was carried out in 43 patients, who were classified into persistent infection group （n = 19） and negative-conversion group （n = 24）. Western-blot was performed to detect serum antibodies to Pmp in all subjects. Results The positivity rate of anti-Pmp antibodies was 90.20% （71/77） in patients, significantly higher than that in the normal controls ［20% （4/20）, P < 0.05］. All the 9 types of anti-Pmp antibodies were detected in patients with a varying positivity rates, which were 61.04% （47/77）, 88.31% （68/77）, 63.63% （49/77）, 28.57% （22/77）, 63.63% （49/77）, 75.32% （58/77）, 62.34% （48/77）, 77.92% （60/77）and 70.13% （54/77） for antibodies against PmpA, PmpB, PmpC, PmpD, PmpE, PmpF, PmpG, PmpH and PmpI respectively. The prevalence was highest for anti-Pmp B antibodies and lowest for anti-Pmp D antibodies. There was no significant difference in the positivity rate of anti-Pmp antibodies between persistent infection group and negative-conversion group. Conclusions Anti-Pmp antibodies could be generated in patients infected with C. trachomatis. The immunogenicity of different Pmps is different, and the immunoprotective activity of Pmps is rather weak. Individual differences exist in serum anti-Pmp antibodies among patients. Nine types of Pmps are expressed in patients with urogenital C. trachomatis infection.

Key words: Chlamydia trachomatis, Polymorphic membrane proteins(Pmps), detection of antibody, immunogenicity