中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (6): 424-425.

• 短篇论著 • 上一篇    下一篇

不同效力糖皮质激素对HaCaT细胞糖皮质激素受体调节的比较

郁博1,何威2,张斌3,杨玲4   

  1. 1. 青岛大学医学院附属医院
    2. 重庆市第三军医大学新桥医院皮肤科
    3. 新桥医院皮肤科
    4. 第三军医大学新桥医院皮肤科
  • 收稿日期:2009-08-10 修回日期:2010-02-03 出版日期:2010-06-15 发布日期:2010-06-08
  • 通讯作者: 郁博 E-mail:yuboskin@126.com
  • 基金资助:

    第三军医大学第二附属医院1520人才基金资助课题

Influences of glucocorticoids with different potency on the expression of glucocorticoid receptors on HaCaT cells: a comparative study

  • Received:2009-08-10 Revised:2010-02-03 Online:2010-06-15 Published:2010-06-08

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摘要：

【摘要】目的 比较三种不同的糖皮质激素对角质形成细胞的糖皮质激素受体调节效应的差异。 方法 将培养的人角质形成细胞HaCaT细胞分为空白对照组、氢化可的松作用组、地塞米松作用组、倍他米松作用组，采用反转录Real-Time PCR和Western blotting法检测各组HaCaT细胞的GRα、GRβ mRNA和蛋白质表达。 结果 三种激素均具有下调HaCaT细胞GRα表达的作用，其中氢化可的松作用最弱，而地塞米松与倍他米松作用之间无明显差异；三种激素均具有上调HaCaT细胞GRβ表达的作用，但此作用之间无明显差异。 结论 不同效力的糖皮质激素对角质形成细胞GRα和GRβ的调节效力不同，可指导临床上正确应用不同效力糖皮质激素外用制剂。

关键词: 糖皮质激素, 糖皮质激素受体, 氢化可的松, 地塞米松, 倍他米松, 角质形成细胞

Abstract:

【Abstract】Objective To compare differences in effects of different glucocorticoids on expression of glucocorticoid receptor in human keratinocyte HaCaT cell line. Methods Cultured HaCaT cells line were divided into hydrocortisone stimulation group and dexamethasone stimulation group and betamethasone stimulation group. The expressions of GRα, β mRNA and protein were examined by Reverse transcription-Real time polymerase chain reaction (RT-PCR) and Western blotting. Results The three kinds of the glucocorticoids with different efficacy could down-regulate the expression of GRα in HaCaT cells line, hydrocortisone had most poorly effect while no difference between dexamethasone with betamethasone exists. The three kinds of glucocorticoid could up-regulate the expression of GRβ in HaCaT cells line，but no difference exists among them. Conclusion The different effects on the expression of GRα and GRβ in keratinocytes by different GC could give guidance for correctly using glucocorticoids with different efficacy.

Key words: Glucocorticoid, Glucocorticoid receptor, Hydrocortisone, Dexamethasone, Betamethasone, Keratinocytes