中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (5): 309-311.

• 论著 • 上一篇    下一篇

沙眼衣原体临床分离株的抗菌药物敏感性及基因分型研究

薛耀华1,郑和平2,杨斌1,周细玉3,吴兴中1,黄进梅4,曾维英4   

  1. 1. 广东省皮肤性病防治中心
    2. 广州市广东省皮肤性病防治中心
    3.
    4. 广州市广东省皮肤病防治中心
  • 收稿日期:2009-11-17 修回日期:2009-12-09 出版日期:2010-05-15 发布日期:2012-04-12
  • 通讯作者: 薛耀华 E-mail:kluk7410@sina.com
  • 基金资助:

    沙眼衣原体对大环内酯类抗生素的耐药性及耐药机制研究

In vitro susceptibility to 6 antimicrobial agents and genotyping of clinical isolates of Chlamydia trachomatis

  • Received:2009-11-17 Revised:2009-12-09 Online:2010-05-15 Published:2012-04-12

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摘要：

目的 探讨广州地区沙眼衣原体临床株对6种抗菌药物的敏感性和基因型分布，以及两者之间的关系。 方法 细胞培养法分离衣原体临床株，测定6种抗菌药物对沙眼衣原体的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。巢式PCR扩增沙眼衣原体主要外膜蛋白基因（omp1）的VS1-2片段，核酸测序分型。结果 346例患者的泌尿生殖道标本接种后，获得76株沙眼衣原体临床分离株，其中40株传代后达到药敏试验所需菌量。药敏试验结果显示克拉霉素、阿奇霉素、罗红霉素、多西环素、四环素和氧氟沙星的MIC50/MIC90（μg/ml）分别为0.008/0.032、0.08/0.16、0.125/0.5、0.032/0.064、0.25/0.5、0.5/1.0。40株共检出7种基因型，其中E型有14株（35％），其次为J型10株（25％）和F型6株（15％）。7种基因型衣原体菌株对阿奇霉素的MIC50完全相同，而罗红霉素、克拉霉素和氧氟沙星的MIC50在不同型别菌株间有1 ~ 4倍的差异。结论 克拉霉素、多西环素和阿奇霉素显示较强的抗衣原体活性，7种基因型衣原体对阿奇霉素显示一致的敏感性。

关键词: 微生物敏感性试验

Abstract:

Objective To assess the vitro susceptibility to 6 antimicrobial agents and genotypes of clinical isolates of Chlamydia trachomatis （Ct） from Guangzhou region. Methods Ct was isolated from clinical specimens by using McCoy cell culture and subjected to propagation. The minimum inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of 6 antimicrobial agents （clarithromycin, roxithromycin, azithromycin, doxycycline, tetracycline, ofloxacin） against Ct isolates were determined in McCoy cell culture. Nested PCR was performed to amplify the outer membrane protein 1 （omp1） VS1-2 gene followed by sequencing. Results Seventy-six Ct strains were isolated from 346 urogenital specimens, and 40 strains met the requirements for susceptibility testing after serial propagation. The MIC50/MIC90 of clarithromycin, azithromycin, roxi-thromycin, doxycycline, tetracycline and ofloxacin were as follows: 0.008/0.032, 0.080/0.160, 0.125/0.500, 0.032/0.064, 0.250/0.500 and 0.500/1.000 mg/L. Seven genotypes were observed. The most prevalent genotypes in decreasing order were E （14, 35%）, J （10, 25%）and F（6, 15%）. The MIC50 was consistent for azithromycin among the 7 genotypes, but varied by 1 - 4 folds for doxycycline, ofloxacin and roxithromycin. Conclusions Clarithromycin, doxycycline and azithromycin exhibit an excellent activity against Ct, and the activity of azithromycin is consistent among the 7 genotypes of Ct.