中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (4): 275-276.

• 短篇论著 • 上一篇    下一篇

角化棘皮瘤的克隆性分析

刘冰梅1,尤海燕2,杨晶3,李黎4,王敏3,尤刚2   

  1. 1. 黑龙江省医院南岗院区
    2. 哈尔滨市黑龙江省医院皮肤科
    3.
    4. 黑龙江省医院
  • 收稿日期:2009-07-06 修回日期:2010-01-29 出版日期:2010-04-15 发布日期:2010-04-07
  • 通讯作者: 刘冰梅 E-mail:liubm555@126.com
  • 基金资助:

    角化棘[皮瘤消退机制及分子生物学的研究

Clonal analysis of keratoacanthoma

  • Received:2009-07-06 Revised:2010-01-29 Online:2010-04-15 Published:2010-04-07
  • Contact: LIU BingMei E-mail:liubm555@126.com

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摘要：

[摘要] 目的 利用磷酸甘油酸激酶（phosphoglycerate kinase, PGK）基因位点克隆性分析技术，分析女性患者X染色体失活的模式，确定角化棘皮瘤(keratoacanthoma, KA)的克隆组成。方法 提取11例女性患者石蜡包埋组织块中的DNA，经甲基化敏感限制内切酶HpaII消化，应用聚合酶链反应（PCR）技术扩增PGK基因的多态性部分，产物经多态性限制内切酶BstXI消化后，琼脂凝胶电泳显示结果。结果 11例KA中，5例有扩增产物表达，其中3例具有BstXI酶切位点的多态性，显示为杂合子，检测结果表明KA为单克隆性。结论 以PCR为基础的PGK克隆性分析技术，可用于确定福尔马林固定、石蜡包埋存档的病理组织的克隆组成。KA为单克隆起源。

关键词: [关键词] 角化棘皮瘤, 克隆, X染色体失活, 磷酸甘油酸激酶

Abstract:

[Abstract] Objective Clonality of keratoacanthoma(KA) was analyzed by means of the polymerase chain reaction（PCR）. Methods Genomic DNA was extracted from archival formalin-fixed, paraffin-embedded tissues and digested through incubation with HpaII and amplified through PCR for a portion of the X-chromosome-linked phosphoglycerate kinase(PGK) gene. The products were treated with BstXI and resolved on agarose gels. Results Amplification was successful in 5 of 11 cases of KA. 3 cases were heterozygous for the BstXI polymorphic site of the PGK amplified product, permitting analysis of clonality. A monoclonal pattern of X-chromosome inactivation was found in 3 informative cases. Conclution PCR amplification can be used for the determination of the pattern of X-chromosome inactivation in formalin-fixed tissues. Such an approach makes it feasible to include specimens from archival tissue collections in the analysis of clonality. KA is of clonal origin.

Key words: [Key word] Keratoacanthoma, Clonality, X chromosome inactivation, phosphoglycerate kinase