中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (1): 22-24.

• 论著 • 上一篇    下一篇

SLE患者外周血CD4+ T淋巴细胞DNA中p16基因启动子区甲基化研究

李政亮1,李遇梅2,马红2,顾文涛3,许辉3,刘丽萍3,吴蔚4,张雪静4   

  1. 1. 漯河医学高等专科学校医疗系
    2. 江苏大学附属医院
    3. 镇江江苏大学附属医院皮肤科
    4. 镇江市江苏大学附属医院
  • 收稿日期:2009-02-18 修回日期:2009-03-11 出版日期:2010-01-15 发布日期:2010-01-05
  • 通讯作者: 李政亮 E-mail:dreamerking1980@163.com
  • 基金资助:

    30771938;国家自然科学基金资助项目（编号）

Methylation state of p16 gene promoter in peripheral CD4+ T cells of patients with systemic lupus erythematosus

  • Received:2009-02-18 Revised:2009-03-11 Online:2010-01-15 Published:2010-01-05

PDF

462

赞

摘要：

目的 检测SLE患者外周血CD4+ T淋巴细胞DNA中p16基因的甲基化状态及其在SLE发病机制中的作用。方法 以Taqman探针为基础的实时定量PCR（Methylight）方法检测28例SLE患者和20例正常人CD4+ T淋巴细胞中p16基因启动子区甲基化状态。结果 SLE患者CD4+ T淋巴细胞的p16基因启动子区甲基化率（35.7％，10/28）高于对照组（10.0％，2/20）。两者比较差异有统计学意义（χ2 = 4.11，P ＜ 0.05）。SLE患者疾病严重程度评分与对应的标准甲基化指数无统计学相关性（rs = -0.29，P > 0.05）。结论 SLE患者CD4+ T淋巴细胞 p16基因甲基化异常，提示p16基因的高甲基化在SLE 的发病中起一定作用。

关键词: 系统性红斑狼疮, CD4+ T细胞, p16, 甲基化

Abstract:

Objective To detect the methylation status of p16 gene promoter in CD4+ T cells of patients with systemic lupus erythematosus （SLE）, and its significance in the pathogenesis of SLE. Methods The p16 gene promoter methylation in peripheral CD4+ T cells was detected with the Taqman probe-based real-time PCR （Methylight） technology in 28 patients with SLE and 20 healthy human controls. Results The methylation rate of p16 gene promoter in peripheral CD4+ T cells was higher in patients with SLE than that in the controls （35.7％ vs 10％, χ2 = 4.11, P ＜ 0.05）. There was no correlation between SLE disease activity index （SLEDAI） and the normalized index of methylation （NIM） of p16 gene promoter （rs = -0.29, P > 0.05）. Conclusion The methylation status of p16 gene promoter is aberrant in CD4+ T cells of SLE patients, suggesting that the hypermethylation of p16 gene plays a certain role in the pathogenesis of SLE.