中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (8): 504-508.

• 论著 • 上一篇    下一篇

基质细胞衍生因子-1及受体CXCR4在阴道念珠菌病小鼠模型中的表达

严小枫 陈宏翔 涂亚庭 刘冬先 陈兴平   

  1. 华中科技大学同济医学院附属同济医院 华中科技大学同济医学院附属协和医院 武汉华中科技大学同济医学院附属协和医院皮肤科 武汉华中科技大学同济医学院附属同济医院皮肤科 武汉同济医科大学附属同济医院皮肤科
  • 收稿日期:2007-12-06 修回日期:2008-03-21 发布日期:2008-08-15
  • 通讯作者: 严小枫 E-mail:doctor.yanxiaofeng@yahoo.com,purples_2004@126.com

Expression of stroma-derived factor 1 and its receptor CXCR4 in a murine model of vaginal candidiasis

Yan Xiaofeng   

  • Received:2007-12-06 Revised:2008-03-21 Published:2008-08-15
  • Contact: Yan Xiaofeng E-mail:doctor.yanxiaofeng@yahoo.com,purples_2004@126.com

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摘要： 目的 检测基质细胞衍生因子-1（SDF-1）及其受体CXCR4在阴道念珠菌病小鼠模型阴道组织及腰引流淋巴结中的表达情况。方法 采用雌激素化阴道念珠菌病小鼠模型，雌性昆明小鼠（80只）随机分四组，A组（雌激素处理感染组）、B组（雌激素处理未感染组）、C组（未用雌激素处理感染组）、正常对照组（5只）。前三组又再分为接种白念珠菌后第2、4、7、14和21天组（各5只）。接种后不同时间点随机取小鼠阴道组织及腰淋巴结，以RT-PCR及免疫组化SABC法检测SDF-1及CXCR4 mRNA及蛋白水平的表达。结果 （1）RT-PCR：与正常小鼠相比，雌激素（B组）对SDF-1及CXCR4 mRNA表达无影响。①小鼠阴道组织：与B组相比，A组小鼠接种后各时间点均见SDF-1 mRNA表达明显增加（F ＝ 114.87，P < 0.01），第14天CXCR4 mRNA明显增加（t = 15.61，P < 0.01）；与正常小鼠相比，C组小鼠接种后第2，4， 7天，SDF-1 mRNA明显增加；第4 ~ 21天CXCR4 mRNA均明显增加。②腰淋巴结：与B组相比，A组小鼠接种后第2天和7 ~ 21天SDF-1 mRNA明显增加（F ＝ 865.62，P < 0.01），CXCR4 mRNA于各时间点表达均增加（F ＝ 161.30，P < 0.01）；与正常小鼠相比，C组小鼠接种后SDF-1 mRNA表达无明显改变（P > 0.05）；CXCR4 mRNA于第7天和14天明显增加（P < 0.05）。（2）免疫组化：A组小鼠阴道黏膜中SDF-1及CXCR4表达在接种后第2天即开始增加，第7天时达高峰，第14天和21天开始下降，但均明显高于对照组（P < 0.05）。结论 感染组小鼠阴道黏膜及腰淋巴结中SDF-1及CXCR4的mRNA及蛋白水平均明显升高。

关键词: 念珠菌病, 外阴阴道;念珠菌, 白色;基质细胞衍生因子-1;CXCR4

Abstract: Objective To examine the expressions of stroma-derived factor 1 （SDF-1） and its receptor CXCR4 in murine vaginal tissue and lumbar lymph nodes during an experimental Candida albicans vaginal infection. Methods A murine model of vaginal candidiasis was established. Eighty female mice were randomly divided into 4 groups, 25 in group A （estrogen-treated mice inoculated with referrence C. albicans strain）, 25 in group B （estrogen-treated mice inoculated with phosphate buffered solution）, 25 in group C （untreated mice infected with C. albicans） and 5 untreated mice free of infection in the control group. The first 3 groups were equally divided into 5 subgroups, including 2-, 4-, 7-, 14- and 21-day groups. The mRNA and protein expression of SDF-1 and CXCR4 in the murine vaginal tissue and draining lumbar lymph nodes was assessed by RT-PCR and immunohistochemistry, respectively, 2, 4, 7, 14 and 21 days after the inoculation of C. albicans. Results No significant difference was observed in the mRNA expression of SDF-1 or CXCR4 between group B and the control group. Compared with group B, the mRNA level of SDF-1 in group A increased both in vaginal tissue from day 2 to 21 （F = 114.87, P < 0.01） and in lumbar lymph nodes on day 2, 7, 14 and 21; increased mRNA expression of CXCR4 was observed in vaginal tissue on day 14 （t = 15.61, P < 0.01） and lumbar lymph nodes from day 2 to 21 （F = 161.30, P < 0.01） in group A. In group C, the mRNA expression of SDF-1 significantly increased in vaginal tissue on day 2, 4 and 7 but remained unchanged in lumbar lymph tissues through the test period （P > 0.05）, and an significant increase was seen in mRNA expression of CXCR4 in vaginal tissue from day 4 to 21 and in lumbar lymph tissues on day 7 and 14 in comparison with the control group （all P < 0.05）. As immunohistochemistry showed, the expression levels of SDF-1 and CXCR4 in vaginal tissue of group A commenced to increase on day 2, reached a peak on day 7, then decreased on day 14 and 21 following the infeciton （P < 0.05）, but they were consistently higher than those of the control group through the study period. Conclusion An increase in mRNA and protein expressions of SDF-1 and CXCR4 is noticed in vaginal tissue and lumbar lymph nodes of C. albicans-infected mice.

Key words: Candidiasis, Vulvovaginal, Candida albicans, Stromal-derived factor 1, CXCR4