中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (2): 87-90.

• 论著 • 上一篇    下一篇

窄波UVB诱导正常皮肤色素斑的研究

张汝芝 朱文元 冯舸 等   

  1. 蚌埠医学院附属医院皮肤科 南京 临床皮肤科杂志编辑部
  • 收稿日期:2007-06-03 修回日期:2007-08-16 发布日期:2008-02-15
  • 通讯作者: 张汝芝 E-mail:ruzhizhang@vip.sina.com,zhangruz@yahoo.com.cn

Narrowband-UVB light induces pigmented maculae on normal human skin

  

  • Received:2007-06-03 Revised:2007-08-16 Published:2008-02-15

摘要: 目的 观测窄波UVB诱导正常人腹部皮肤色素沉着斑的颜色变化指标。方法 采用窄波UVB治疗仪,以1.5倍的最小红斑量照射30例志愿者的腹部皮肤,形成人工色素沉着斑。在照射前、照射后1,2,4,6,8周,分别用 CM-2600d分光光度计测量局部皮肤的L*、a*、b*、△L、△a、△b、 △E值,以SIAscopy仪检测色素斑处血红蛋白、胶原蛋白、黑素总量,并捕获相应图像。同时,用数码相机拍摄志愿者腹部照片。结果 照射后局部皮肤呈淡灰色,4 h后出现局部潮红,1周后色素沉着最明显,2例受试者照射部位出现细小脱屑,所有受试者均无水疱发生。CM-2600d分光光度计测量照射部皮肤,结果显示,照射后1周,L*下降到最低,a*值升至最高,b*变化不明显。1周后L*值开始升高,而a*值下降,直到照射后8周,各自接近照射前水平。SIAscopy仪检测提示,照射后1周,皮肤血红蛋白增加最明显,以后迅速下降,低于照射前水平。而总黑素含量和胶原蛋白照射后持续缓慢增加,第6周达最高。结论 窄波UVB可诱导正常皮肤色素斑形成,通过观察皮肤色素的变化规律,客观地评估美白产品的功效。

Abstract: Objective To observe the changes in chromacity parameters of pigmented maculae induced by narrowband-UVB light on the normal abdominal skin of healthy volunteers. Methods Minimal erythematous doses (MED) were determined in 30 healthy female volunteers. In order to induce the formation of pigmented macules, the abdominal skin of these volunteers was irradiated with 1.5 folds of MED of narrowband-UVB light. Before and after irradiation, spectrophotometer CM-2600d was employed to detect the skin chromacity parameters, including L* (representing the relative lightness ranging from total black to total white), a* (giving the balance between red and green), b* (denoting the balance between yellow and blue), △L, △a, △b and △E values (total color difference), and SIAscopy to quantify the levels of hemoglobin, collagen protein and total melanin in the pigmented macules. The corresponding images were captured by a computer. At the same time, the pigmented macules on the abdomen were recorded by a digital camera. Results After irradiation, the skin immediately turned to light-grey, then flared up 4 hours later; maximum pigmentation was observed at the end of the first week. Tiny desquamation was observed in 2 subjects, but no blisters were found in any of the volunteers. As shown by spectrophotometer CM-2600d, L* value reached to the nadir, a* value to the apex, while b* value remained unchanged by week 1. Since then, L* and a* values changed in opposite directions until week 8, when both values approximated the original levels before the irradiation. The hemoglobin level experienced a significant increase within the first week after the irradia- tion, then descended rapidly with time to a level lower than that before the irradiation. On the other hand, the levels of collagen protein and total melanin continued to increase and peaked on week 6 after the irradiation. Conclusions Narrowband-UVB light could induce the formation of pigmented macules on normal skin. The efficacy of skin whitening products can be evaluated by observing the changes in skin pigmentation.