中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (10): 663-665.

• 论著 • 上一篇    下一篇

微小染色体维持蛋白-2在正常及良性增生和恶性增生皮损中的表达

张晓光 李艳玲 王生 等   

  1. 石家庄河北医科大学第二医院皮肤科
  • 收稿日期:2007-11-16 修回日期:2008-01-23 发布日期:2008-10-15
  • 通讯作者: 张晓光 E-mail:zhangxiaoguang078@163.com

Expression of minichromosome maintenance 2 protein in normal skin as well as lesions of malignant hyperplasia and non-malignant hyperplasia

  

  • Received:2007-11-16 Revised:2008-01-23 Published:2008-10-15

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摘要： 目的 探讨微小染色体维持蛋白-2（MCM-2）在皮肤恶性增生皮损、皮肤良性增生皮损、正常皮肤中表达的强度及分布特点。方法 免疫组化SP法检测MCM-2蛋白在皮肤恶性增生皮损 （皮损1组）、皮肤良性增生（皮损2组）、不同部位的正常皮肤（正常对照组）中的表达。结果 在皮损1组，MCM-2蛋白在基底层及其以上有表达。在皮损2组，MCM-2蛋白在基底层表达，基底层以上偶见。正常对照组MCM-2蛋白仅在基底层表达。秩和检验得出：MCM-2蛋白在皮损1组表皮细胞中的表达强度高于皮损2组，皮损2组的表达强度高于正常对照组，均有统计学意义（P < 0.05）。χ2检验得出，MCM-2蛋白阳性细胞比例在皮损1组基底细胞高于皮损2组和正常对照组，差异均有统计学意义（均为P < 0.05）。MCM-2蛋白阳性细胞比例在皮损2组基底细胞高于正常对照组，差异有统计学意义（P < 0.05）。结论 MCM-2蛋白能比较客观地反映表皮细胞增殖规律的生物学特性，表皮细胞的增殖状态不同，MCM-2蛋白的表达也不同。

关键词: MCM－2蛋白,增生, MCM-2蛋白 分布 表达 细胞

Abstract: Objective To detect the expression intensity and distribution of minichromosome maintenance 2 protein （MCM-2） in normal skin and lesions of malignant and non-malignant hyperplasia. Methods Three groups of samples were collected, i.e., malignant group （including 15 cases of Bowen disease or highly differentiated squamous cell carcinoma of Grade Ⅰ or Ⅱ）, non-malignant group （including 4 cases of chromomycosis, 2 cases of sporotrichosis, 5 cases of seborrheic keratosis, 4 cases of verruca vulgaris, 4 cases of chronic eczema, 4 cases of cutaneous fibroma）, and normal group （10 cases of normal human control）. The distribution and intensity of MCM-2 expression in the epidermis of these samples were assessed by immunohistochemical SP method. Results The expression of MCM-2 was observed in basal and superbasal layer of epidermis in lesions of malignant and non-malignant hyperplasia, and only in epidermal basal layer in normal skin. A significant increment was observed in the density of MCM-2 positive cells in superbasal layer in malignant lesion compared with the non-malignant lesion. The epidermal expression level of MCM-2 in the non-malignant lesion was significantly lower than that in the malignant lesion, but higher than that in the normal skin （μ = -2.529, -3.705, respectively, both P < 0.05）; the same was true for the proportion of MCM-2-postive basal cells. Conclusions The expression of MCM-2 protein varies with the proliferation status of epidermal cells, and may serve as an objective marker for epidermal cell proliferation.