中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (9): 536-538.

• 论著 • 上一篇    下一篇

甲氧沙林对黑素细胞粘附及基质金属蛋白酶-2的影响

张宪旗1, 郑敏1, 牟宽厚2, 冯捷3, 马慧群3   

  1. 1. 浙江大学医学院附属第二医院皮肤科, 杭州 310009;
    2. 西安交通大学第一医院皮肤科;
    3. 西安交通大学第二医院皮肤科
  • 收稿日期:2006-12-04 出版日期:2007-09-15 发布日期:2007-09-15
  • 通讯作者: 冯捷, email:fengjie@medmail.com.cn E-mail:fengjie@medmail.com.cn
  • 基金资助:
    国家自然科学基金(30200247)

In vitro effects of 8-methoxypsoralen on the adhesion and matrix metalloproteinase-2 expression of melanocytes

ZHANG Xian-qi1, ZHENG Min1, MOU Kuan-hou2, FENG Jie3, MA Hui-qun3   

  1. Department of Dermatology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China
  • Received:2006-12-04 Online:2007-09-15 Published:2007-09-15

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摘要： 目的 探讨甲氧沙林治疗白癜风的可能机制.方法 正常人黑素细胞来自包皮环切术切取的包皮,用包被纤维黏连蛋白的48孔培养板检测黑素细胞粘附,用带微孔膜的Transwell培养板研究黑素细胞迁移,用RT-PCR方法检测基质金属蛋白酶-2(MMP-2)mRNA表达.结果 50nmol/L和100nmol/L甲氧沙林可促进黑素细胞粘附(P<0.05)和迁移(P<0.05),100nmol/L对黑素细胞促迁移作用最强(P<0.01),而且10nmol/L甲氧沙林即可明显促进MMP-2mRNA表达(P<0.01).在一定浓度下,甲氧沙林促黑素细胞粘附、迁移和MMP-2mRNA表达呈剂量依赖性,在不同浓度组间比较差异均有统计学意义(P<0.01、P<0.05和P<0.01).结论 甲氧沙林通过促进MMP-2的表达来促进黑素细胞粘附和迁移.

关键词: 黑素细胞, 甲氧沙林, 明胶酶A

Abstract: Objective To investigate the possible mechanism of 8-methoxypsoralen in the treatment of vitiligo.Methods Human melanocytes were obtained from normal human foreskins,and 48-well culture dishes coated with fibronectin were used to perform melanocyte adhesion assay.Motility was assessed using the Transwell micropore filter method.RT-PCR was employed to measure MMP-2 expression.Results The 8-methoxypsoralen at 50 and 100 nmol/L could significantly promote melanocyte adhesion and migration (P<0.05),with the best work concentration at 100 nmol/L.The expression of MMP-2 mRNA was significantly increased by 8-methoxypsoralen at 10 nmol/L only(P<0.05).Within a certain range of concentration,8-methoxypsoralen exerted its functions in a dose dependent manner(P<0.01 for adhesion,P<0.05 for migration,P<0.01 for MMP-2 expression).Conclusions The efficacy of 8-methoxypsoralen in the treatment of vitiligo may be partially due to its promoting effects on melanocyte adhesion and migration,and the effect on migration may be carried out partly by enhancing the expression of MMP-2.

Key words: Melanocytes, Methoxypsolen, Gelatinase A