中华皮肤科杂志

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芪白合剂对培养的人黑素细胞生长及酪氨酸酶、MITF基因表达的影响

李剑1, 傅雯雯1, 付文焕2, 张勇1, 祝绿川1, 项蕾红1, 郑志忠1, 乔伟伟3   

  1. 1. 复旦大学附属华山医院皮肤科, 上海, 200040;
    2. 复旦大学附属华山医院药剂科, 上海, 200040;
    3. 复旦大学实验动物部
  • 收稿日期:2006-09-18 出版日期:2007-07-15 发布日期:2007-07-15
  • 通讯作者: 傅雯雯,email:fuww@medmail.com.cn E-mail:fuww@medmail.com.cn

Effects of Qibai-mixture on the proliferation and mRNA expression of tyrosinase and microphthalmia transcription factor in cultured human melanocytes

LI Jian1, FU Wen-wen1, FU Wen-huan2, ZHANG Yong1, ZHU Lü-chuan1, XIANG Lei-hong1, ZHENG Zhi-zhong1, QIAO Wei-wei3   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2006-09-18 Online:2007-07-15 Published:2007-07-15

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摘要： 目的 研究芪白合剂对黑素细胞生长及黑素细胞酪氨酸酶、小眼相关转录因子(MITF)基因表达的影响.方法 芪白合剂组方黄芪、党参、白芷、白蒺藜、鸡血藤,水煎煮浓缩成3g/mL,取青紫蓝兔,按12mL·kg-1·d-1连续灌胃3天后于末次给药1h,2h和5h无菌采血,收集血清;采用体外培养正常人黑素细胞,观察不同采血时间的芪白合剂含药血清对黑素细胞生长、酪氨酸酶的活性、黑素合成以及用RT-PCR的方法测定其对酪氨酸酶、MITF基因表达的影响.结果 含药血清与对照血清比较,能明显促进黑素细胞生长,上调酪氨酸酶的活性和黑素合成(P<0.05),以1h和2h所采血清作用明显.并可以促进酪氨酸酶和MITF的基因表达.结论 芪白合剂可以通过直接作用于黑素细胞和酪氨酸酶通道来促进黑素合成.

关键词: 黑素细胞, 一元酚单氧酶, 芪白合剂, MITF

Abstract: Objective To investigate the effects of Qibai-mixture on the proliferation,tyrosinase activity and melanogenesis of cultured human melanocytes.Methods The water decoction of Qibai-mixture, which consisted of huangqi,dangshen,baizhi,baijili,jixueteng,was concentrated to 3 g/mL,then was used to treat black,purple and blue rabbits by intragastric administration at a dose of 12 mL·kg-1·d-1 for three days.Serum samples were collected from the rabbits at 1 h,2 h and 5 h alter the last intragastric administration.Human melanocytes were cultured with the serum samples or drug-free serum as control for 24 or 48 h.The proliferation,tyrosinase activity and melanogenesis of melanocytes were detected by MTT method,DOPA-oxidation,and NaOH-assay,respectively.The mRNA expression of tyrosinase and microphthalmia transcription factor(MITF)was examined with semiquantitative RT-PCR.Results Compared with the control serum,the serum collected at 1 h and 2 h after the last administration could significantly stimulate the proliferation,up-regulate the activity of tyrosinase,promote the melaningenesis, and increase the mRNA expression of tyrosinase and MITF,of the cultured melanocytes.Conclusion These results demonstrate that Qibai-mixture could promote melanogenesis by directly acting on melanocytes and tyrosinase signal pathway.

Key words: Melanocytes, Monophenol monooxygenase, QIBAI-mixture, MITF