耐伊曲康唑烟曲霉临床分离株对四种抗真菌药物的敏感性测定

中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (12): 722-724.

耐伊曲康唑烟曲霉临床分离株对四种抗真菌药物的敏感性测定

刘伟, 乔建军, 万结, 陈伟, 王端礼, 李若瑜

北京大学第一医院皮肤性病科, 北京大学真菌和真菌病研究中心 100034

收稿日期:2007-01-29 出版日期:2007-12-15 发布日期:2018-09-29
基金资助:
国家自然科学基金(30500027);教育部留学归国人员启动基金

Antifungal susceptibility testing of clinical isolates of itraconazole-resistant Aspergillus fumigatus to four antifungal drugs

LIU Wei, QIAO Jian-jun, WAN Zhe, CHEN Wei, WANG Duan-li, LI Ruo-yu

Department of Dermatology and Venereology, Peking University First Hospital, Peking University Research Center for Medical Mycology, Beijing 100034, China

Received:2007-01-29 Online:2007-12-15 Published:2018-09-29

摘要/Abstract

摘要： 目的测定耐伊曲康唑炯曲霉临床株对4种抗真菌药物的敏感性.方法自一例对伊曲康唑无效的肺曲霉球患者体内系列分离6株烟曲霉.利用美国临床实验室标准化研究所(CLSI)的微量液基稀释法M38-A方案和E-test法测定6株烟曲霉临床分离株对两性霉素B、卡泊芬净、米卡芬净、伏立康唑和伊曲康唑的敏感性.结果 6株烟曲霉中,2株对伊曲康唑的最低抑菌浓度(MIC)为0.5μg/mL,其他4株的MIC均>16μg/mL;两性霉素B和伏立康唑对6株烟曲霉的MIC分别为1μg/mL和0.25～1μg/mL,卡泊芬净和米卡芬净对6株烟曲霉的最低有效浓度均≤0.03μg/mL.E-test法测定结果也显示,卡泊芬净和伏立康唑对6株烟曲霉有良好的抑制活性.结论耐伊曲康唑烟曲霉临床株对两性霉素B、卡泊芬净、米卡芬净和伏立康唑敏感.

关键词: 曲霉菌, 烟, 伊曲康唑, 抗药性, 真菌

Abstract: Objective To evaluate the susceptibility of itraconazole-resistant Aspergillus fumigatus isolates to four other antifungal drugs.Methods Six strains of Aspergillus fumigatus were serially isolated from a patient with lung aspergilloma,who was resistant to itraconazole therapy.Clinical Laboratory Standard Institute broth microdilution method M38-A and E-test were applied to test the susceptibility of A.fumigatus strains to amphotericin B,itraconazole,voriconazole,caspofungin and micafungin.Results With M38-A method,the minimal inhibitory concentration(MIC)of itraconazole was 0.5μg/mL for 2 strains and more than 16μg/mL for the other 4 strains;the MICs of amphotericin B and voriconazole were 1μg/mL and 0.25-1μg/mL respectively for all the 6 strains;and the minimal effective concentration(MEC)of caspofungin and micafungin were no more than 0.03μg/mL for all the 6 strains.Moreover,E-test results also revealed that caspofungin and voriconazole had a good antifungal activity against all the strains,including the 4 itraconazole-resistant ones.Conclusion The itraconazole-resistant A.fumigatus strains isolated from a patient with lung aspergilloma are susceptible to amphotericin B,voriconazole,caspofungin,and micafungin.

Key words: Aspergillus fumigatus, Itraconazole, Drug resistance, fungal

刘伟, 乔建军, 万结, 陈伟, 王端礼, 李若瑜. 耐伊曲康唑烟曲霉临床分离株对四种抗真菌药物的敏感性测定[J]. 中华皮肤科杂志, 2007, 40(12): 722-724.

LIU Wei, QIAO Jian-jun, WAN Zhe, CHEN Wei, WANG Duan-li, LI Ruo-yu. Antifungal susceptibility testing of clinical isolates of itraconazole-resistant Aspergillus fumigatus to four antifungal drugs[J]. Chinese Journal of Dermatology, 2007, 40(12): 722-724.

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