中华皮肤科杂志

• 论著 • 上一篇    下一篇

姜黄素对小鼠黑素瘤的抑制作用及对核因子κB激活和生存素表达的影响

刘彦群, 周琳, 魏志平   

  1. 徐州医学院附属医院皮肤性病科, 江苏省221002
  • 收稿日期:2006-11-14 出版日期:2007-11-15 发布日期:2007-11-15

Curcumin inhibits mouse melanoma growth and influences nuclear factorκB activation and survivin expression

LIU Yan-qun, ZHOU Lin, WEI Zhi-ping   

  1. Department of Dermatology and Venereology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu, China
  • Received:2006-11-14 Online:2007-11-15 Published:2007-11-15

PDF (PC)

179

赞

摘要： 目的 探讨姜黄素抑制小鼠黑素瘤作用的可能机制.方法 将黑素瘤细胞株B16F10接种于小鼠右大腿外侧皮下,建立荷黑素瘤小鼠模型,于接种后第7天分别予以50和100 mg/kg姜黄素干预,以0.5 mL无血清RPMI 1640培养基腹腔注射为对照组,观察不同处理组瘤体的变化,采用免疫印迹方法检测肿瘤组织核因子κB(NF-κB)p65表达程度,应用半定量逆转录聚合酶链反应方法检测生存素mRNA的水平.结果 姜黄素治疗组小鼠瘤重均较肿瘤对照组降低,尤以50 mg/kg姜黄素组为著,抑瘤率可达55.56%(P<0.01);治疗组肿瘤组织NF-κBp65表达水平均较肿瘤对照组降低,50 mg/kg姜黄素组降低63.19%,100 mg/kg姜黄素组降低55.74%,治疗组与肿瘤对照组差异有统计学意义(P<0.01);治疗组生存素mRNA均较肿瘤对照组降低,50 mg/kg组降低51.02%,100mg/kg组降低34.69%,治疗组与肿瘤对照组差异有统计学意义(P<0.01);肿瘤组织NF-κB活性与生存素mRNA表达水平呈正相关(r=0.81,P<0.01).结论 姜黄素可能通过抑制NF-κB活化从而下调其下游生存素基因表达来抑制恶性黑素瘤的生长.

关键词: 黑色素瘤, 实验性, 姜黄素, 转录因子RelA, 生存素

Abstract: Objective To investigate the possible mechanism of curcumin inhibition to murine melanoma growth.Methods Melanoma cell line B16F10 was injected subcutaneously into the outer side of mouse right thigh to establish a melanoma-bearing mouse model.Seven days after the establishing the model, these mice were treated with intraperitoneal injection of curcumin at 50 and 100 mg/kg respectively or RPMI 1640 culture medium as control.Fourteen days later,the mice were killed,tumor weight was calculated;the tumor nuclear factorκB activity and survivin mRNA expression were measured by Western blot and RT-PCR,respectively.Results The tumor weight was significantly lower in the curcumin-treated mice than that in the controls (P<0.01),with an inhibition rate of 55.56 % by 50 mg/kg of curcumin.The level of NF-κB activity and survivin mRNA expression were decreased by 63.19% and 51.02%,respectively,by curcumin of 50 mg/kg,55.74% and 34.69%,respectively,by curcumin of 100 mg/kg (all P<0.01),as compared with the culture medium.There was a positive correlation between the tumor NF-κB activity and survivin mRNA expression(r=0.81,P<0.01).Conclusion Curcumin may inhibit the growth of malignant melanoma by suppressing NF-κB activation and down-regulating survivin gene expression.

Key words: Melanoma,experimental, Curcumin, Transcription factor RelA, Survivin