中华皮肤科杂志

• 论著 • 上一篇    下一篇

UVB诱导皮肤免疫抑制和表没食子儿茶素没食子酸酯光保护作用

徐丽贤, 骆丹, 吉玺, 林向飞, 朱洁, 缪旭   

  1. 南京医科大学第一附属医院皮肤科, 210029
  • 收稿日期:2005-11-16 出版日期:2006-04-15 发布日期:2006-04-15
  • 通讯作者: 骆丹,email:daniluo2005@yahoo.com.cn E-mail:daniluo2005@yahoo.com.cn
  • 基金资助:
    国家自然科学基金(30371294)

Skin immunosuppression by UVB irradiation and photoprotection by(-)-epigallocatechin-3-gallate

XU Li-xian, LUG-Dan, JI Xi, LIN Xiang-fei, ZHU Jie, MIAO Xu   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2005-11-16 Online:2006-04-15 Published:2006-04-15

PDF (PC)

116

赞

摘要： 目的 探讨表没食子儿茶素没食子酸酯(EGCG)对不同剂量中波紫外线(UVB)慢性辐射BALB/c小鼠皮肤的保护作用。方法 EGCG局部外用于小鼠背部皮肤后予不同强度UVB辐射,每日1次,连续1个月,RT-PCR法检测IFN-γ和IL-10 mRNA的表达水平变化。结果 不同剂量UVB慢性辐射后小鼠皮肤中IFN-γmRNA表达水平较对照组降低(P<0.01),IL-10 mRNA表达水平较对照组升高(P<0.01),并与UVB辐射强度呈一定剂量依赖关系。EGCG处理后可影响UVB辐射诱导上述细胞因子表达(P<0.01)。结论 UVB辐射下调IFN-γ和上调IL-10转录水平,可能与UVB辐射诱导局部免疫抑制有关。

关键词: 紫外线, 干扰素Ⅱ型, 白细胞介素10, 表没食子儿茶素没食子酸酯

Abstract: Objective To investigate the(-)-epigallocatechin-3-gallate(EGCG)photoprotection in BALB/c mouse skin damaged by chronic irradiation of different doses of ultraviolet B(UVB).Methods BLAB/c mice were pretreated with topical EGCG on the back,followed by daily irradiation with different dosages of UVB.One month later,skin samples were obtained and detected for IFN-γand IL-10 mRNA expression by RT-PCR.Results Compared with the control group,IFN-γmRNA expression in the skin of BALB/c mice was significantly decreased,while IL-10 mRNA expression was significantly increased after chronic UVB irradiation(both P<0.01),both in a dose-dependent manner.Topical application of EGCG had a significant influence(P<0.01)on the mRNA expression of IFN-γand IL-10 induced by UVB irradiation.Conclusions UVB irradiation could down-regulate IFN-γmRNA expression but up-regulate IL-10 mRNA expression in BALB/c mice skin.EGCG has a potential photo-protective effect on UVB induced local immunosuppression in BALB/c mouse skin.

Key words: Ultraviolet rays, Interferon typeⅡ, Interleukin-10, (-)-epigallocatechin-3-gallate