中华皮肤科杂志

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系统性白念珠菌病患者血清中芽管抗体的检测

钱丽洁, 朱敏, 李莉, 朱利平, 章强强, 王家俊   

  1. 复旦大学附属华山医院皮肤科 上海 200040
  • 收稿日期:2005-09-26 出版日期:2006-04-15 发布日期:2006-04-15

Measurement of antibodies to germ tube of Candida albicans in the sera of patients with systemic candidiasis

QIAN Li-jie, ZHU Min, LI Li, ZHU Li-ping, ZHANG Qiang-qiang, WANG Jia-jun   

  1. Departmeat of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2005-09-26 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨一种诊断深部白念珠菌病的新方法.方法 以间接免疫荧光法测定白念珠菌系统感染患者血清中芽管抗体的滴度,并与常规真菌检测结果及菌株磷脂酶活性相比较.结果 血清中芽管抗体检测与常规检测方法(镜检+培养)阳性率比较,有较好的一致性(P<0.001).磷脂酶活性测定结果显示,感染组患者分离株较定植组的磷脂酶活力差异有统计学意义(P<0.05);磷脂酶活性与芽管抗体滴度具有相关性,P<0.001.结论 检测白念珠菌芽管抗体在系统件白念珠菌感染诊断中具有一定的诊断意义.

关键词: 念珠菌病, 念珠菌, 白色, 抗体, 真菌

Abstract: Objective To establish a new method to diagnose deep candidiasis.Methods The titre of antibody to germ tube of Candida albicans in sera was detected by indirect immunofluorescent antibody(IFA)assay,then the result was compared with that of microcopic examination and culture of samples,and phospholipase activity of isolates.Results The positive ratio obtained by measurement of antibody correlated well(P<0.01)with that by the routine diagnostic method(microscopy+culture).The Pz value of extracellular phospholipase was significantly lower(P<0.05)in the infection group(both direct microscopic examination and culture were positive)than in the colonization group(direct microscopy was negative but culture was positive).The activity of extracellular phospholipase was associated(P<0.001)with the titre of antibody to germ tube in serum.Conclusion Detection of antibodies to germ tube of Candida albicans may be used to diagnose systemic candidiasis.

Key words: Candidiasis, Candida albicans, Antibodies,fungal