中华皮肤科杂志

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拉坦前列素对体外培养的猪毛囊生长的影响

张卉, 李晓凤, 范卫新   

  1. 南京医科大学第一附属医院(江苏省人民医院)皮肤科 210029
  • 收稿日期:2005-11-11 出版日期:2006-04-15 发布日期:2006-04-15
  • 通讯作者: 范卫新, email:fanweixi@jlonline.com E-mail:email:fanweixi@jlonline.com
  • 基金资助:
    国家自然科学基金(30170860)

Effect of latanoprost on pig hair follicles in vitro

ZHANG Hui, LI Xiao-feng, FAN Wei-xin   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2005-11-11 Online:2006-04-15 Published:2006-04-15

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摘要： 目的探讨拉坦前列素对体外培养的猪毛囊生物学特性的影响。方法采用猪毛囊体外培养模型,记录体外培养的猪毛囊生长速度和毛球部形态学改变,评价拉坦前列素对毛发生长的作用。结果与阴性对照组比较,拉坦前列素质量浓度达到0.8ng/mL时即能使培养的猪毛囊生长速度加快(P<0.05),并持续生长达8d,且毛囊毛球部仍然呈现生长期样形态,而对照组则表现为退行期/休止期样外观。结论拉坦前列素对体外培养的猪毛囊生长具有明显的促进作用。能够延长毛囊的生长期。

关键词: 毛囊, 组织培养技术, 拉坦前列素, 米诺地尔, 生长调节素类

Abstract: Objective To investigate the effect of latanoprost on the growth of pig hair follicles in vitro.Methods A culture model of pig hair follicles was applied.The morphological changes in the hair bulbs and growth speed of the hair follicles cultured with different concentrations (0.032,0.16,0.8,4,20,100,500,2500 ng/mL) of latanoprost were observed and recorded on day 1,2,4,6,8 in the culture.Resttlts Compared with the follicles without latanoprost,those cultured with the drug (0.8,4,20 ng/mL) grew more rapidly (P<0.05).And on day 8 the follicular bulbs treated with latanoprost (0.8,4,20 ng/mL) were still in an anagen-phase,while those without the drug exhibited a catagen/teiogen-phase appearance.Conclusion The study shows that certain concentrations of latanprost could stimulate the growth of cultured pig hair follicles,which may be due to prolonging of the anagen phase in hair cycle.

Key words: Hair follicle, Tissue culture techniques, Latanoprost, Minoxidil, Somatomedins