中华皮肤科杂志

• 论著 • 上一篇    下一篇

血小板膜糖蛋白及抗血小板抗体在过敏性紫癜患者中的表达

阮黎明, 刘晓艳, 刘国英, 李明伟   

  1. 浙江大学医学院附属第一医院皮肤科 杭州 310003
  • 收稿日期:2005-11-16 出版日期:2006-04-15 发布日期:2006-04-15

Expression and significance of platelet membrane glycoproteins and antiplatelet antibody in patients with anaphylactoid purpura

RUAN Li-ming, LIU Xiao-yan, LIU Guo-ying, LI Ming-wei   

  1. Department of Dermatology, First Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310003, China
  • Received:2005-11-16 Online:2006-04-15 Published:2006-04-15

摘要: 目的 探讨过敏性紫癜患者抗血小板抗体、血小板膜糖蛋白表达量的变化及其临床意义。方法 以单克隆抗体为探针,采用流式细胞仪检测45例过敏性紫癜患者血小板α颗粒膜糖蛋白(CD62P)、溶酶体颗粒膜糖蛋白(CD63)、血小板膜表面糖蛋白(CD41)的表达水平;同时采用ELISA法检测抗血小板抗体水平。结果 过敏性紫癜患者急性期CD62P、CD63阳性百分数及抗血小板抗体表达水平明显高于缓解期和对照组,差异有统计学意义,CD41表达水平无明显变化。紫癜肾炎型CD62P、CD63及CD41和抗血小板抗体表达水平与混合型比较,差异无统计学意义。紫癜肾炎型及混合型与单纯皮肤型比较,CD62P高于后者,差异有统计学意义;CD63、抗血小板抗体差异无统计学意义。结论 过敏性紫癜患者存在体液免疫异常。血小板活化与过敏性紫癜病程变化关系密切。

关键词: 紫癜, 过敏性, 血小板膜糖蛋白类, 抗血小板抗体

Abstract: Objective To investigate the expression and significance of platelet membrane glycoproteins and antiplatelet antibody in patients with anaphylactoid purpura.Methods Forty-five patients with anaphylactoid purpura were divided into three groups according to their clinical manifestations,15 in dermal purpura group,18 in mixed group and 12 in nephritis group.The expression of CD62P,CD63 and CD41 in these 45 patients was detected by flow cytometry.The level of antiplatetlet antibody was analysed by ELISA with monoclonal antibody as the probe.Results The percentage of CD62P,CD63 and the level of antiplatelet antibody in patients in acute phase were significantly higher than those in regressive phase and those in normal control.Neither of the expression of CD62P,CD63 and CD41,nor the level of antiplatetlet antibody in nephritis group was significantly different from that in the mixed group.Compared with dermal purpura group,the percentage of CD62P was significantly higher in the nephritis group and mixed group.There was no significant difference in CD41 expression among all groups.Conclusion Abnormal humoral immunity exists in anaphylactoid purpura.The activation of platelet could be closely related to the progress of anaphylactoid purpura.CD62P and CD63 could be considered as an index for monitoring of symptoms,determination of prognosis and predicting the outcome in anaphylactoid purpura.

Key words: Purpura,Henoch-Schonlein, Platelet membrane glycoproteins, Antiplatelet antibody