中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (10): 602-604.

• 论著 • 上一篇    下一篇

干扰素γ释放试验诊断药疹的作用

江佳1, 翁孟武2   

  1. 1. 复旦大学附属上海市第五人民医院皮肤科;
    2. 复旦大学附属华山医院皮肤科
  • 收稿日期:2005-12-30 出版日期:2006-10-15 发布日期:2006-10-15

Diagnostic role of interferon-γ release test in drug eruption

JIANG Jia1, WENG Meng-wu2   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200046, China
  • Received:2005-12-30 Online:2006-10-15 Published:2006-10-15

PDF

109

赞

摘要： 目的 探讨干扰素γ(IFN-γ)释放试验在诊断药疹及寻找可疑致敏药物中的作用.方法 取患者静脉血约5mL,肝素抗凝后梯度离心分离出淋巴细胞,加入受试药物,在37℃、5% CO2条件下孵育24h后离心收集上清液,-70℃保存,最后用ELISA方法测定上清液中的IFN-γ含量.经公式计算出IFN-γ释放增加百分数,与不加药物的空白对照相比,增加20%以上为阳性结果.结果 试验组10例药疹患者,IFN-γ释放试验结果阳性8例,阳性率80.0%;对照组9例,阳性1例,两组比较,P<0.005,差异有统计学意义.试验的敏感性和特异性分别为88.9%和80%.试验组10例根据可疑致敏药物的不同共进行了13种可疑药物试验,阳性9种,阳性率为69.2%,与对照组(1/9)相比,P<0.05,差异有统计学意义.试验的敏感性和特异性分别为90%和66.7%.结论 本试验的阳性率、敏感性均高于以往报道的其他药疹体外实验,提示IFN-γ释放试验对于明确药疹的诊断及探讨致敏药物有着一定的作用.

关键词: 药疹, IFN-γ释放试验

Abstract: Objective To investigate the diagnostic role of IFN-γ release test in drug eruption.Methods Lymphocytes from heparinized venous blood were separated by gradient centrifugation,cultured in test tubes at 37℃ in 5% CO2 with each tested drug,or with medium alone.After 24 hours of incubation,these test tubes were centrifuged,supematants were collected and stored in -70℃until the detection of human IFN-γ by ELISA in these samples was performed.The increase percentage of IFN-γ release in the supernatants of peripheral blood lymphocytes was calculated according to a formula in which the increase percentage obtained from a drug test was subtracted by that obtained from the blank control test;if the result was more than 20%,the IFN-γ release test was defined as positive.Results The positivity rate of IFN-γ release test was higher in patients with drug eruption than in the control group [80% (8/10) vs 11% (1/9),P<0.005].The sensitivity and specificity of the IFN-γ test were 88.9% and 80% respectively.According to the suspected sensitizing drugs,13 IFN-γ tests were performed for the l0 patients.Of the 13 tests,9 were positive with a positivity rate of 69.2%,which was much higher than that in the control group (P<0.05).Conclusion The positivity rate and sensitivity of the test were both higher than those of other previous in vitro tests for drug eruption,which suggests that IFN-γ release test plays a role in the confirmation of diagnosis of drug eruption,and in the investigation of sensitizing drugs.

Key words: Drug eruptions, IFN-γ release test