中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (1): 7-9.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者白介素10受体cDNA基因多态性研究

姚煦, 李遇梅, 胡兹嘉, 李文忠, 陈志强   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2005-01-05 发布日期:2006-01-15
  • 基金资助:
    国家自然科学基金资助项目(30170863)

Polymorphism within the interleukin-10 receptor cDNA gene in patients with systemic lupus erythematosus

YAO Xu, LI Yu-mei, HU Zi-jia, LI Wen-zhong, CHEN Zhi-qiang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2005-01-05 Published:2006-01-15

PDF

112

赞

摘要： 目的 探讨白介素10受体(IL-10R)的两个基因位点G241A和G520A基因多态性与系统性红斑狼疮(SLE)患者发病的相关性.方法 采用聚合酶链反应-单链构象多态性(PCR-SSCP)和聚合酶链反应限制性片段多态性(PCR-RFLP)及DNA测序方法进行基因分型.结果 IL-10R2基因型与SLE易感性显著相关.G520/G520基因型增加SLE发病危险性,P=0.004,OR=0.515,95%的可信区间为0.444-0.579;G520/A520基因型增加SLE发病危险性,P=0.055,OR=1.968,95%的可信区间为0.981-3.949.IL-10R1基因型与SLE易感性无显著相关.各个基因型组合的分布情况和优势比显示具有IL-10R1 G241/G241基因型和IL-10R2 G520/G520的个体SLE发病危险性明显高于其他的基因型组合,P=0.004,OR=0.515,95%的可信区间为0.444-0.597.结论 IL-10R2基因型与SLE易感性显著相关,G520/G520基因型增加SLE发病危险性.具有IL-10R1 G241/G241基因型和IL-10R2 G520/G520的个体SLE发病危险性明显高于其他的基因型组合.

关键词: 红斑狼疮,系统性, 受体,白细胞介素, 多态性现象,单链构象

Abstract: Objective To assess the association between polymorphism within the interleukin-10 receptor cDNA gene (IL-10R) and Chinese patients with systemic lupus erythematosus (SLE).Methods The IL-10R genotypes of 94 SLE patients and 80 healthy subjects were examined by reverse transcription-polymerase chain reaction-single strand conformation polymorphism method (RT-PCR-SSCP),RT-PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing.Results There were significant differences in the IL-10R2 genotype frequencies of these two groups.The IL-10R2 G520/G520 genotype increased the risk of developing SLE (OR=0.515,95% CI 0.414-0.579,P=0.004) and individuals who had G520/A520 genotype also had a higher susceptibility to SLE (OR=1.968,95% CI 0.981-3.949,P=0.055).There was no significant association between SLE and IL-10R1 genotypes.The risk of developing SLE was detected in the individuals who had the combination of IL-10R1 G241/G241 and IL-10R2 G520/G520 (OR=0.515,95% CI 0.444-0.597,P=0.004).Conclusion The IL-10R2 genotypes of G520/G520 and G520/A520 as well as the combination of genotypes IL-10R1G241/G241 and IL-10R2 G520/G520 may increase the susceptibility to SLE in Chinese people.

Key words: Lupus erythematosus,systemic, Receptors,interleukin, Polymorphism,single-stranded conformational