中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 533-535.

• 论著 •    下一篇

PCR-RFLP检测皮肤分枝杆菌

李晓杰, 王洪生, 吴勤学, 崔盘根, 刘训荃   

  1. 中国医学科学院中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:2004-09-07 发布日期:2005-09-15

Development of PCR-RFLP for Identification of Eight Mycobacteriai Species

LI Xiao-jie, WANG Hong-sheng, WU Qin-xue, CUI Pan-gen, LIU Xun-quan   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Received:2004-09-07 Published:2005-09-15

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摘要： 目的 建立检测8种常见皮肤分枝杆菌的PCR-RFLP方法。方法 ①用通用引物对分枝杆菌的热休克蛋白hsp65基因序列进行PCR扩增:②用限制性内切酶BstEⅡ和HaeⅢ对扩增产物进行消化,根据所得片段确定各分枝杆菌特征性的酶切图谱,从而对分枝杆菌进行鉴别。结果 8种常见皮肤分枝杆菌感染标准株(鸟、胞内、堪萨斯、结核、瘰疬、海、偶发、龟分枝杆菌)的酶切图谱与文献报道一致。结论 PCR-RFLP方法可用以检测鉴定皮肤分枝杆菌。

关键词: 分枝杆菌, 结核, 鸟, 非典型性

Abstract: Objective To develop a PCR-RFLP method for the identification of eight mycobacterial species. Methods PCR was performed targeting the gene encoding 65-kDa heat shock protein which was common to all mycobacteria. Two restriction enzymes, BstE Ⅱ and Hae Ⅲ, were used to digest the PCR products, and specific restriction patterns of different mycobacteria were obtained. Results The specific restriction patterns of different mycobacteria were identical to the data previously reported. Conclusion We could differentiate M. avium, M. intracellulare, M. kansasii, M. tuberculosis, M. scrofulaceum, M. marinum, M. fortuitum and M. chelonae in one experiment by PCR-RFLP.

Key words: Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium, atypical