中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (6): 380-382.

• 论著 • 上一篇    下一篇

外用维A酸引起小鼠皮肤反应的研究

吴秋菊, 马鹏程, 陈志强, 魏峻   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2004-07-02 出版日期:2005-06-15 发布日期:2005-06-15
  • 通讯作者: 马鹏程,E-mail:maidcams@public1.ptt.js.cn E-mail:maidcams@public1.ptt.js.cn

Skin Reactions Induced by Topical Retinoic Acid in Mice

WU Qiu-ju, MA Peng-cheng, CHEN Zhi-qiang, WEI Jun   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2004-07-02 Online:2005-06-15 Published:2005-06-15

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摘要： 目的 观察在动物实验中外用0.1%全反式维A酸霜及其基质引起的皮肤反应。方法 小鼠背部每天分别外用0.1%全反式维A酸霜及其基质,连续使用30d,肉眼观察皮肤变化,并用生物学仪器进行红斑和皮屑检测。结果 外用0.1%全反式维A酸霜,肉眼观察第3天左右出现红斑、脱屑反应,第6天左右达高峰,第12天左右消失,继续用药至第30天,未见反应再现;仪器测得红斑值在第1天时已明显升高,第6天时达最高峰,并持续高峰状态至第9天,第11天时降至基本正常,至第30天,均维持在正常范围,皮屑指数在用药第3天时明显升高,第9天时达高峰,然后逐渐下降,第27天时降至正常。外用基质部位与用药前比无差异。结论 在动物实验中,外用0.1%全反式维A酸霜引起的皮肤反应在不停药的情况下,呈现“出现-高峰-消退”的一过性规律。维A酸皮肤反应与基质无关。

关键词: 皮肤刺激试验, 投药,皮肤, 动物替代试验, 维甲酸

Abstract: Objective To investigate the skin reactions induced by to pical 0.1% tretinoin and its vehicle in mice. Methods 0.1% tretinoin and its vehicle were applied separately on mice backs,once a day for 30 days.The skin reactions,including erythema and scaling,were observed macroscopically and meas ured by biological instruments. Results Macroscopically the skin reactions of erythema and scaling induced by topical tretinoin occurred on day 3 after application,reached its peak on day 6 and disappeared on day 12,and neither erythema nor abnormal scaling appeared in spite of continuing application until day 30.The erythema value measured was enhanced significantly on day 1 after application,reached its peak on day 6,maintained until day 9 and became normal on day 11.From then to day 30,it maintained at the normal level.The scaling value measu red enhanced significantly on day 3 after application,reached its peak on day 9 and became normal on day 27.Topical application of the vehicle induced neither skin reaction nor any change of erythema and scaling value. Conclusions Topical application of 0.1% tretinoin induces skin reaction characterized by erythema and scaling.The reaction,transient but not related to the vehicle,experiences a process of "occurrence-peak-disappearance".

Key words: Tretinoin, Skin irritancy tests, Administration, cutaneo us, Animal testing alternatives