中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (6): 374-376.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者CCR7+CD8+CD45RO+T细胞诱导CD4+T细胞向Th2分化

陈朗, 吴春晨, 何玉玲, 谢珞琨, 周钢, 谭锦泉   

  1. 武汉大学医学院免疫系 430071
  • 收稿日期:2004-07-17 出版日期:2005-06-15 发布日期:2005-06-15
  • 通讯作者: 谭锦泉,E-mail:jinquan_tan@hotmail.com E-mail:jinquan_tan@hotmail.com
  • 基金资助:
    国家自然科学基金资助项目(39870674)

CCR7+CD8+CD45RO+ T Cells induced Differentiation of CD4+ T Cells to Th2 Cells in Active Systemic Lupus Erythematosus

CHEN Lang, WU Chun-chen, HE Yu-ling, XIE Luo-kun, ZHOU Gang, TAN Jin-quan   

  1. Department of Immunology, Medical Institute of Wuhan University, Wuhan 430071, China
  • Received:2004-07-17 Online:2005-06-15 Published:2005-06-15

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摘要： 目的 探讨系统性红斑狼疮(SLE)患者外周血CCR7+CD8+CD45RO+记忆性T细胞对CD4+T细胞的诱导分化作用及其与SLE发病的关系。方法 流式细胞仪、实时定量RT-PCR和RNA印迹检测同系CCR7+CD8+CD45RO+T细胞和树突细胞协同刺激CD4+T细胞分泌细胞因子。结果 活动期SLE患者CCR7+CD8+CD45RO+记忆性T细胞诱导CD4+T细胞表达Th2类细胞因子:白介素4的表达效率显著高于正常人对照组和非活动期SLE患者组(P<0.01),1型调节性T细胞(Tr1)源性细胞因子:白介素10和转化生长因子β的表达效率均低于正常对照组和非活动期SLE患者组(P<0.01);而活动期和非活动期SLE患者干扰素γ的表达效率显著低于正常人对照组(P<0.01)。结论 活动期SLE患者外周血CCR7+中央型记忆性T细胞可与树突细胞相互作用,诱导同系CD4+T细胞向Th2分化,发挥CCR7-CD45RO+效应性记忆性T细胞的功能。

关键词: CD8阳性T淋巴细胞, 红斑狼疮,系统性, CD4阳性T淋巴细胞, 白细胞介素4, 白细胞介素10, 转化生长因子β, 干扰素II型

Abstract: Objective To determine the functions of CCR7+CD8+CD45RO+ T cells on CD4+T cells in systemic lupus erythematosu(SLE). Methods The expression of cytokines in CD4+T cells was measured by flow cytometry,real-time quantitative reverse transcription polymerase chain reaction and Northern blotting.A chemotaxis assay was used to detect their functions. Results In the case of active SLE,CCR7+CD8+CD45RO+T cells could induce CD4+T cells to express high levels of Th2-cytokine (IL-4),and lowlevels of Tr1-cytokines (IL-10 and TGF-β),than those in normal controls and inactive SLE (P<0.01).The levels of Th1-cytokine (IFN-γ) were lower in active and inactive SLE than those in normal controls (P<0.01). Conclusions In the case of active SLE,CCR7+ central memory T cells may interact with dendritic cells,and induce differentiation of syngeneic CD4+T to Th2 cells.

Key words: Interleukin-4, Interleukin-10, Transforming growth factor beta, Interferon typeⅡ, CD4-positive T-lymphocytes, Lupus erythematosus, systemic, CD8-positive T-lymphocytes