中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (6): 365-367.

• 论著 • 上一篇    下一篇

重组腺病毒介导金黄色葡萄球菌肠毒素A基因在小鼠B16黑素瘤细胞中的表达

牟霞1, 陆洪光1, 余德厚1, 吴承龙2, 汪宇1   

  1. 1. 贵阳医学院附属医院皮肤科 550004;
    2. 贵阳医学院微生物教研室 550004
  • 收稿日期:2004-05-20 出版日期:2005-06-15 发布日期:2005-06-15
  • 通讯作者: 陆洪光,E-mail:hongguanglu@hotmail.com E-mail:hongguanglu@hotmail.com
  • 基金资助:
    国家自然基金资助项目(D-39960071)

The Expression of Staphylococcal Enterotoxin A(SEA) Gene on Murine B16 Melanoma Cells Mediated by Replication-defective Recombinant Adenovirus Vector

MU Xia1, LU Hong-guang1, YU De-hou1, WU Cheng-long2, WANG Yu1   

  1. Department of Dermatology, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China
  • Received:2004-05-20 Online:2005-06-15 Published:2005-06-15

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摘要： 目的 研究金黄色葡萄球菌肠毒素A(SEA)基因通过重组腺病毒介导转染入B16黑素瘤细胞中的表达情况。方法 用RT-PCR和流式细胞仪检测SEA基因在B16黑素瘤细胞中的表达。结果 在转染SEA基因的B16黑素瘤细胞中检测到SEA基因的转录,并于转染后24h开始有SEA蛋白的表达,到72h表达量达最高值87.22,之后减弱,直到168h仍有少许表达。结论 SEA基因可以在B16黑素瘤细胞中表达,为下一步黑素瘤的基因治疗奠定基础。

关键词: 肠毒素类, 基因表达, 黑色素瘤,实验性

Abstract: Objective To investigate staphylococcal enterotoxin A (SE A) gene expression on murine B16 melanoma cells mediated by replication-defective recombinant adenovirus. Methods SEA expression on the B16 melanoma cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM). Results RT-PCR and FCM proved that SEA could specifically express on the B16 melanoma cells. Conclusion The successful expression of SEA gene on the B16 melanoma cells might be used for the targeted treatment of mali gnant melanoma.

Key words: Gene expression, Melanoma, experimental, Enterotoxins