中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (6): 351-353.

• 论著 • 上一篇    下一篇

特应性皮炎患者趋化因子及其受体的研究

钱起丰1, 林立航2, 张明霞1, 刘启文3   

  1. 1. 深圳市皮肤病防治研究所, 性病防治研究中心 518020;
    2. 广东医学院皮肤性病科;
    3. 深圳市儿童医院皮肤科
  • 收稿日期:2004-08-07 出版日期:2005-06-15 发布日期:2005-06-15
  • 基金资助:
    广东省医学科研基金资助项目(A2003713)

Detection of Serum Chemokines and Their Receptors on Peripheral Blood CD4+ T Lymphocytes in Patients with Atopic Dermatitis

QIAN Qi-feng1, LIN Li-hang2, ZHANG Ming-xia1, LIU Qi-wen3   

  1. Shenzhen Institute of Dermatology, Center for STD Control and Research, Shenzhen 518020, China
  • Received:2004-08-07 Online:2005-06-15 Published:2005-06-15

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摘要： 目的 探讨几种重要的趋化因子及其受体的表达在特应性皮炎(AD)发病中的作用。方法 采用酶联免疫吸附试验检测39例AD患者及正常人血清中γ干扰素诱导蛋白-10(IP-10)、基质细胞衍生因子-1α(SDF-1α)、嗜酸粒细胞趋化因子、胸腺和活化调节的趋化因子(TARC)及巨噬细胞来源的趋化因子(MDC)等水平;同时用流式细胞仪分析外周血CD4+T细胞表面趋化因子受体CXCR3、CXCR4、CCR3、CCR4及CCR5的表达。结果 与正常人对照组相比,AD患者血清SDF-1α、TARC和MDC水平显著升高(P<0.001),IP-10及嗜酸粒细胞趋化因子水平则无明显改变(P>0.05),外周血CXCR3、CCR3、CCR4及CCR5在CD4+T细胞表达水平显著增加(P<0.001);血清TARC和MDC水平的变化与疾病严重程度相关(r分别为0.669及0.409,P分别为<0.001及<0.01)。结论 具有生物活性趋化因子及其受体介导的T细胞和嗜酸/嗜碱粒细胞的聚集、激活后释放的炎性介质在AD发病中起着重要作用。

关键词: 皮炎,特应性, 趋化因子类, 受体,趋化因子

Abstract: Objective To study the role of serum chemokines and their receptors in the pathogenesis of atopic dermatitis(AD). Methods Serum levels of interferon γ-inducible protein-10(IP-10),stromal cell-derived factor-1α (SDF-1α),eotaxin,thymus and activation-regulated chemokine(TARC) as well as macrophage-derived chemokine (MDC) were detected by enzyme-linked immunosor bent assay(ELISA) in 39 patients with AD and 39 normal controls.Meanwhile,chem okine receptors CXCR3,CXCR4,CCR3,CCR4 and CCR5 on peripheral blood CD4+T-lymp hocytes were analyzed by flow cytometry with a two-color immunofluorescentstaining in 39 patients with AD. Results Serum levels of SDF-1α,TARC and MDC were significantly higher(P<0.001) in patients with AD[(1361.5±352.5) pg/mL,(807.1±606.7) pg/mL and (1290.4±494.6) pg/mL,respectively] than those in the normal controls[(834.9±223.6) pg/mL,(308.5±183.0) pg/mL and (811.8±196.6) pg/mL,respectively].There was no significant difference in serum levels of IP-10 and eotaxin (P>0.05) between the patients[(123.6±110.4) pg/mL and (68.7±26.2) pg/mL,respectively]and controls [(100.7±73.7) pg/mL and(66.8±20.5) pg/mL,respectively].The expression of CXCR3,CCR3,CCR4 and CCR5 on CD4+T cells was increased (P<0.001) in peripheral blood of the patients (87.2%±10.2%,15.2%±13.8%,55.9%±9.9% and 7.4%±4.5%,respectively) in comp arison with those of the healthy controls (65.4%±10.2%,6.1%±5.5%,47.2%±8.2% and 2.3%±0.4%,respectively).Serum TARC and MDC levels were associated with the disease activity(r=0.669 and 0.409,respectively;P<0.001 and P<0.01,respectively). Conclusions Both chemokines with biological activity and their receptors maylead to the recruitment and activation of Tlymphocytes,eosinophils and basophils,which appear to play an important role in the pathogenesis of AD by releasing mediators of inflammation.

Key words: Dermatitis, atopic, Chemotactic factors, Receptors, chem okine