中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (5): 300-302.

• 论著 • 上一篇    下一篇

皮肤混合瘤中bcl-2和增殖细胞核抗原的免疫组化研究

尤德渊1, 王艳东1, 尤海燕2   

  1. 1. 哈尔滨医科大学第二附属医院皮肤科, 150086;
    2. 黑龙江省医院皮肤性病研究中心
  • 收稿日期:2004-05-27 出版日期:2005-05-15 发布日期:2005-05-15

Immunohistochemical Study on Bcl-2 and PCNA in Mixed Tumor of Skin

YOU De-yuan1, WANG Yan-dong1, YOU Hai-yan2   

  1. Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China
  • Received:2004-05-27 Online:2005-05-15 Published:2005-05-15

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摘要： 目的 探讨bcl-2和增殖细胞核抗原(PCNA)在皮肤混合瘤中的表达及意义。方法 采用免疫组化S-P法及免疫组化双染技术对12例皮肤混合瘤皮损标本中bcl-2和PCNA的表达进行观察。结果 12例患者的皮损标本均见bcl-2及PCNA阳性染色。肿瘤巢内,衬于囊腔和导管样结构内侧的肿瘤细胞示PCNA阳性,而上皮样肿瘤细胞示bcl-2阳性。bcl-2阳性的肿瘤细胞多呈PCNA阴性染色,而bcl-2阴性的肿瘤细胞多呈PCNA阳性染色。结论 皮肤混合瘤中bcl-2、PCNA的异常表达似与此肿瘤的发生相关。

关键词: 增殖细胞核抗原, 皮肤混合瘤, 原癌基因蛋白质bcl-2

Abstract: Objective To investigate the expression of bcl-2 and PCNA in mixed tumor of skin. Methods Bcl-2 and PCNA expression in 12 cases of mixed tumor of skin were studied using immunohistochemical S-P method and double-staining. Results Positive reactions of bcl-2 and PCNA were observed in all cases. PCNA staining was positive in tumor cells lining cystic and duct-like structures in tumor nests, but bcl-2 staining was positive in epitheloid tumor cells. Most tumor cells with bcl-2 were PCNA negative and vice versa. Conclusion Aberrant expression of bcl-2 and PCNA in mixed tumor of skin may play a role in tumorigenesis.

Key words: Mixed tumor of skin, Proto-oncogene proteins bcl-2, Proliferating cell nuclear antigen