中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (5): 294-296.

• 论著 • 上一篇    下一篇

HSPC016基因在凝集性生长人毛乳头细胞的表达

杨卫兵, 郝飞, 宋志强, 杨希川   

  1. 第三军医大学西南医院皮肤科, 重庆400038
  • 收稿日期:2004-05-24 出版日期:2005-05-15 发布日期:2005-05-15
  • 通讯作者: 郝飞,E-mail:haofei@mail.tmmu.com.cn E-mail:haofei@mail.tmmu.com.cn
  • 基金资助:
    国家自然科学基金资助项目(30300249)

Gene HSPC016 Expressed in Aggregated Human Dermal Papilla Cells of Hair Follicles

YANG Wei-bing, HAO Fei, SONG Zhi-qiang, YANG Xi-chuan   

  1. Department of Dermatology, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China
  • Received:2004-05-24 Online:2005-05-15 Published:2005-05-15

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摘要： 目的 确定造血干细胞相关基因HSPC016的表达与毛乳头细胞凝集性生长的关系。方法 对凝集性生长的人毛乳头细胞、非凝集性生长的人毛乳头细胞和人真皮成纤维细胞分别进行细胞内mRNA原位杂交;用RT-PCR从以上3组细胞总RNA扩增HSPC016基因。结果 原位杂交发现HSPC016基因在凝集性生长的人毛乳头细胞表达,而在非凝集性生长的人毛乳头细胞和人真皮成纤维细胞均不表达;RT-PCR可以从凝集性生长的人毛乳头细胞RNA中扩增到约200bp目的片段,而不能从非凝集性生长人毛乳头细胞和人真皮成纤维细胞中扩增到相应目的片段。结论 HSPC016基因在凝集性生长的人毛乳头细胞特异性表达,可能与毛乳头细胞的分化和功能状态有关。

关键词: 细胞培养, 基因表达, 造血干细胞相关基因

Abstract: Objective To identify the relationship between expression of hematopoeitic stem/progenitor cell(HSPC)-related gene HSPC016 and aggregative behavior of the dermal papilla cells of hair follicles. Methods The aggregated human dermal papilla cells, non-aggregated human dermal papilla cells and the human dermal fibroblasts were used in this study. Expression of HSPC016 mRNA was investigated in the three cell groups by intracellular mRNA hybridization in situ and RT-PCR. Results By in situ hybridization, it was shown that gene HSPC016 specifically expressed in the aggregated dermal papilla cells, but not in the non-aggregated human dermal papilla cells and human dermal fibroblasts. A 200bp fragment of target cDNA was amplified from RNAs of the aggregated human dermal papilla cells by RT-PCR, but could not from RNAs of other two cell lines. Conclusions Gene HSPC016 was specifically expressed in the aggregated dermal papilla cells, and expression of HSPC016 might be related to the differentiation and functions of dermal papilla cells.

Key words: Cell culture, Gene expression, Hematopoeitic stem/progenitor cell-related gene