中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (5): 279-281.

• 论著 • 上一篇    下一篇

连接酶链反应检测性病患者沙眼衣原体感染

郑和义, 曹经江   

  1. 中国医学科学院、中国协和医科大学北京协和医院皮肤性病科, 北京100730
  • 收稿日期:2004-05-15 出版日期:2005-05-15 发布日期:2005-05-15

Detection of Chlamydia trachomatis Infection by Ligase Chain Reaction in STD Patients

ZHENG He-yi, CAO Jing-jiang   

  1. Department of Dermatology and Venereology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China
  • Received:2004-05-15 Online:2005-05-15 Published:2005-05-15

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摘要： 目的 评价连接酶链反应(LCR)诊断性病患者尿道/宫颈中沙眼衣原体(Ct)的意义。方法 STD门诊尿道(宫颈)炎患者276例,取尿道/宫颈拭子,以LCR分析法检测Ct。采用每4份标本相混合的方法分别以LCR分析法检测尿道/宫颈拭子标本中的Ct,其中56例患者同时进行尿道/宫颈拭子Ct细胞培养。差异性结果由PCR法扩增Ct的主要外膜蛋白基因来进行确认,确定LCR分析法检测Ct的敏感性、特异性。结果 LCR分析法检测尿道/宫颈拭子Ct的敏感性、特异性分别为96.7%和100%。采用每4份标本相混合的方法分别以LCR分析法检测尿道/宫颈拭子标本中的Ct,与单独用每份标本逐一进行LCR检测比较,结果完全一致,符合率为100%。结论 以尿道/宫颈拭子为标本,LCR分析法检测Ct的敏感性、特异性高。适用于诊断泌尿生殖道Ct感染。用标本相混合的方法LCR分析检测尿道/宫颈拭子标本中的Ct,适用于Ct感染的普查。

关键词: 衣原体,沙眼, 性传播疾病

Abstract: Objective To evaluate the performance of ligase chain reaction(LCR) in the detection of Chlamydia trachomatis(Ct) from urethral or cervical samples in STD patients. Methods Ct was detected by LCR in urethral or cervical swabs from 276 cases with urethritis or cervicitis attending our STD clinic. All 276 urethral or cervical swab specimens were pooled by 4 into 69 pools for detection. Chlamydial cell culture was performed in 56 cases with nongonococal urethritis or cervicitis. Discrepant results were analyzed by PCR with primers targeting Ct major outer membrane protein gene. Results The sensitivity and specificity of LCR assay were 96.7% and 100% respectively. Two LCR approaches, pooling or individual testing, yielded 100% of consistency in the detection of Ct in urethral or cervical specimens. Conclusions LCR provides a highly sensitive and specific assay for detection of Ct from urethral and cervical samples, and could be recommended for the diagnosis of genital chlamydial infection. Pooling LCR is suitable for screening of urethral or cervical Ct infection in population study.

Key words: Sexually transmitted diseases, Chlamydia trachomatis