中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (5): 273-275.

• 论著 • 上一篇    下一篇

尖锐湿疣患者外周血树突细胞亚群的检测

钱起丰   

  1. 深圳市皮肤病防治研究所, 性病防治研究中心, 518020
  • 收稿日期:2004-05-19 出版日期:2005-05-15 发布日期:2005-05-15

Peripheral Blood Dendritic Cell Subsets in Patients with Condyloma Acuminata

QIAN Qi-feng   

  1. Shenzhen Institute of Dermatology, Center for STD Control and Research, Shenzhen 518020, China
  • Received:2004-05-19 Online:2005-05-15 Published:2005-05-15

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摘要： 目的 检测尖锐湿疣患者外周血树突细胞(DC)亚群并探讨其意义。方法 采用免疫荧光三标记流式细胞仪检测25例尖锐湿疣患者外周血DC亚群(DC1/CD11c+和DC2/CD123+),并以26例正常人作为对照。结果 尖锐湿疣患者外周血CD123+DC百分数为(0.63±0.34)%比对照组(0.41±0.17)%明显增高(P<0.01)。长病程组及复发患者CD123+DC增高明显(均为P<0.01),而短病程组及初发患者则无明显改变(均为P>0.05)。尖锐湿疣患者组CD11c+DC百分数(1.40%±0.44%)与对照组(1.57%±0.78%)相比差异无统计学意义(P>0.05)。结论 尖锐湿疣患者外周血DC2亚群占优势,导致向Th2样免疫反应偏移,此可能与尖锐湿疣的复发和病程的长短有关。

关键词: 树突细胞, 尖锐湿疣

Abstract: Objective To determine the peripheral blood dendritic cell (DC) subsets in patients with condyloma acuminata (CA) and its significance. Methods The CD11c+ peripheral blood DC (DC1) and CD123+ DC(DC2) subsets were analyzed by flow cytometry with three-color immunofluorescent staining in 25 patients with CA and 26 healthy controls. Results The proportion of CD123+ peripheral blood DCs was significantly increased in patients with CA[(0.63±0.34)%]in comparison with that in healthy controls [(0.41±0.17)%](P<0.01). It was further found that CD123+ DCs were increased markedly in the patients with long-course of disease [(0.83±0.42)%](P<0.01) and recurrent groups [(0.74±0.36)%](P<0.01) respectively, in comparison with patients with short-course of disease [(0.50±0.20)%] and first attack [(0.47±0.24)%](P<0.05). There was no significant difference of CD11c+ DC proportions between patients [(1.40±0.44)%]and the controls[(1.57±0.78)%](P>0.05). Conclusions The predominance of CD123+ DCs in peripheral blood of CA may favor Th2-dominated immunity, which may be related to recurrence and course of the disease.

Key words: Condylomata acuminata, Dendritic cells