中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (5): 271-272.

• 论著 • 上一篇    下一篇

咪喹莫特对BALB/c小鼠细胞因子生成的影响

周小勇, 李新宇, 郑家润   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:2004-05-29 出版日期:2005-05-15 发布日期:2005-05-15

Influence of Imiquimod on Cytokine Induction in BALB/c Mice

ZHOU Xiao-yong, LI Xin-yu, ZHENG Jia-run   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China.
  • Received:2004-05-29 Online:2005-05-15 Published:2005-05-15

摘要: 目的 探讨咪喹莫特的免疫调节机制。方法 ELISA检测经咪喹莫特灌胃(30mg/kg)后不同时间点的BALB/c小鼠血清干扰素α(IFN-α)、白介素12(IL-12)、干扰素γ(IFN-γ)、白介素4(IL-4)浓度的变化。ELISA检测喂食咪喹莫特的BALB/c小鼠脾细胞在与0.25μg/mL刀豆素A共孵育时分泌IFN-γ、IL-4的能力。结果 咪喹莫特体内诱导BALB/c小鼠生成IFN-α、IL-12,在诱导后2h达高峰;咪喹莫特体内无诱导BALB/c小鼠生成IFN-γ、IL-4的作用;接受咪喹莫特灌胃的BALB/c小鼠脾细胞体外受ConA诱导时,分泌IFN-γ和IL-12的能力增强。结论 咪喹莫特诱导BALB/c小鼠分泌IFN-α、IL-12等前炎症因子,并由此促进机体获得性免疫。

关键词: 白细胞介素12, 白细胞介素4, 干扰素α, 动物替代试验, 咪喹莫特

Abstract: Objective To study the immunomodulating mechanisms of imiquimod. Methods BALB/c mice were fed with imiquimod(30 mg/kg). After 0.5, 1, 2, 4 hours, serum concentrations of IFN-α, IL-12, IFN-γ, IL-4 were measured by ELISA. The spleen cells of the mice were incubated with 0.25 μg/mL of ConA. IFN-γ and IL-4 were detected by ELISA in the supernatant. Results IL-12 and IFN-α could be induced by imiquimod in vivo. Serum levels of IL-12 and IFN-α peaked in 2 hours after feeding with imiquimod. Serum IFN-γ and IL-4 seemed not to be induced significantly by imiquimod. In vitro, expression of IFN-γ and IL-12 in spleen cells taken from mice fed with imiquimod was enhanced by co-incubation with 0.25 μg/mL ConA. Conclusions Imiquimod could induce the pro-inflammatory cytokine secretion and promote the acquired immunity in BALB/c mice.

Key words: imiquimod, Interleukin-12, Interleukin-4, Interferon-alpha, Animal testing, alternative