中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (10): 615-618.

• 论著 • 上一篇    下一篇

外阴鳞状上皮内瘤变皮损中人乳头瘤病毒16、18癌基因整合性研究

王秀丽1, 廖康煌1, Peter Hillemanns2   

  1. 1. 复旦大学附属华山医院皮肤科 200040;
    2. 德国Munich University
  • 收稿日期:2004-11-25 发布日期:2005-10-15

Integration of HPV 16/18 DNA in Vulvar Intraepithelial Neoplasia

WANG Xiu-li1, LIAO Kang-huang1, Peter Hillemanns2   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2004-11-25 Published:2005-10-15

摘要: 目的 探讨人乳头瘤病毒(HPV)16、18在外阴鳞状上皮内瘤变(VIN)皮损中的感染和其癌基因在人基因组中的整合情况。方法 采用捕获杂交法及PCR技术筛选高危型HPV和HPV16、18DNA阳性VIN标本:经RT-PCR、巢式PCR及DNA杂交研究HPV16、18DNA阳性VIN标本中的HPV基因转录。结果 32例VIN患者皮损中24例(75%)为高危型HPV阳性,23例为HPV16阳性,1例为HPV18阳性。23例HPV16阳性标本中除1例VIN Ⅱ皮损无HPV转录外,15份标本为游离体型HPV16基因转录,其余7例VINⅢ标本呈现整合型HPV 16癌基因转录;1例HPV18阳性VINⅢ标本发现为整合型HPV18癌基因转录。结论 大部分VINⅡ、Ⅲ标本存在HPV16感染,HPV癌基因的整合多发生于VINⅢ的皮损;推测高危型HPV癌基因在人基因组中的整合与VIN的发生及其向外阴鳞状细胞癌的发展有关。

关键词: 乳头状瘤病毒, 人, 外阴鳞状上皮内肿瘤, 病毒整合

Abstract: Objective To investigate the pathogenic role of high risk HPVs and genomic integration of HPV 16/18 DNA in vulvar intraepithelial neoplasia (VIN) patients. Methods Hybrid Capture HPV DNA assay and PCR were performed to detect high-risk HPVs and HPV 16/18 positive samples. Then RT-PCR, nested PCR and Southern blotting analysis were used in HPV 16/18 positive samples for the amplification and analysis of papillomavirus oncogene transcripts. Results In 32 cases of VIN patients, 24(75%) high-risk HPVs were detected, 23 were HPV 16, and only 1 was HPV 18. Genomic integration of HPV 16/18 was observed in 8 cases of VIN Ⅲ (7 HPV 16 and 1 HPV 18). Except 1 case of VIN Ⅱ, 15 of 23 HPV-16 positive specimens displayed HPV16 episomal transcripts, 7 displayed integrated transcripts. Conclusions HPV 16 is positive in most cases of VIN Ⅱ and VIN Ⅲ. Integration of HPV 16/18 oncogene only occurs in VIN Ⅲ. It is concluded that the integration of high-risk HPVs oncogene is related with the occurrence, and the development to vulvar cancer, of VIN.

Key words: Papillomavirus, human, Vulvar intraepithelial neoplasia, Virus integration