人类免疫缺陷病毒确认试验假阳性问题及其对策

中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (4): 245-246.

人类免疫缺陷病毒确认试验假阳性问题及其对策

刘全忠¹, 蒋岩²

1. 天津医科大学总医院皮肤性病科 300052;
2. 中国疾病预防控制中心性病艾滋病预防控制中心

收稿日期:2003-07-22 出版日期:2004-04-15 发布日期:2004-04-15

刘全忠¹, 蒋岩²

1. 天津医科大学总医院皮肤性病科 300052;
2. 中国疾病预防控制中心性病艾滋病预防控制中心

Received:2003-07-22 Online:2004-04-15 Published:2004-04-15

摘要/Abstract

摘要： 人类免疫缺陷病毒(HIV)感染的标准检测方法是用免疫学方法检测HIV抗体.这种检测涉及两个步骤:先用酶免疫法(EIA)筛查(HIV-1/2),随后用特异性的蛋白印迹实验予以确定.目前,各国采用的HIV确认试验绝大多数是蛋白印迹.蛋白印迹试验中的特异性条带是HIV或HIV前病毒的各种成分引发的特异性免疫反应.然而,直到20世纪90年代早期,蛋白印迹操作没有标准化,至少有3种不同的蛋白印迹操作流程广泛用于HIV的实验诊断中,包括筛查和确认.

刘全忠, 蒋岩. 人类免疫缺陷病毒确认试验假阳性问题及其对策[J]. 中华皮肤科杂志, 2004, 37(4): 245-246.

刘全忠, 蒋岩. [J]. Chinese Journal of Dermatology, 2004, 37(4): 245-246.

参考文献

[1] Dodd RY, Fang CT. The western immunoblot procedure for HIV antibodies and its interpretation. Arch Pathol Lab Med,1990, 114:240-245.
[2] Cordes RJ, Ryan ME. Pitfalls in HIV testing. Application and limitations of current tests. Postgrad Med, 1995, 98:177-180.
[3] Healey DS, Bolton WV. Apparent HIV-1glycoprotein reactivity on western blot in uninfected blood donors. AIDS, 1993, 7:655-658.
[4] Sayre KR, Dodd RY, Tegtmeier G, et al.False-positive human immunodeficiency virus type 1 western blot tests in noninfected blood donors. Transfusion, 1996,36:45-52.
[5] Kleinman S, Busch MP, Hall L, et al.False-positive HIV-1 test results in a lowrisk screening setting of voluntary blood donation. Retrovirus Epidemiology Donor Study. JAMA, 1998, 280:1080-1085.
[6] Busch MP, Satten GA, Herman SA, et al.HIV plasma RNA for confirmation of HIV-1 p24 Ag/anti-HIV reactivity in seroconverting donors. Transfusion, 1996, 36:41.
[7] Schwartz DH, Mazumdar A, Winston S, et al. Utility of various commercially available human immunodeficiency virus (HIV) antibody diagnostic kits for use in conjunction with efficacy trials of HIV-1vaccines. Clin Diagn Lab Immunol, 1995,2:268-271.
[8] Ghosh K, Javeri KN, Mohanty D, et al.False-positive serological tests in acute malaria. Br J Biomed Sci, 2001, 58:20-23.
[9] Esteva MH, Blasini AM, Ogly D, et al.False positive results for antibody to HIV in two men with systemic lupus erythematosus. Ann Rheum Dis, 1992, 51:1071-1073.
[10] Myrmel H, Haukenes G. False-positive band in the gp41 region in anti-HIV western blotting. APMIS, 1988, 96:950-951.
[11] Simonsen L, Buffington J, Shapiro CN, et al. Multiple false reactions in viral antibody screening assays after influenza vaccination. Am J Epidemiol, 1995, 141:1089-1096.
[12] Walensky RP, Rosenberg ES, Ferraro MJ,et al. Investigation of primary human immunodeficiency virus infection in patients who test positive for heterophile antibody.Clin Infect Dis, 2001, 33:570-572.
[13] Centers for Disease Control and Prevention. Update:HIV-2 infection among blood and plasma donors-United States, June 1992-June 1995. MMWR, 1995, 42:603-606.
[14] Centers for Disease Control and Prevention. Identifieation of HIV-1 Group O infection-Los Angeles County, California,1996. MMWR, 1996, 45:561-564.
[15] Migali E, Mariotti D, Lovari A, et al.HIV-1:absence of infection in subjects with indeterminate western blot. Allergol Immanopathol (Madr), 1993, 21:61-65.
[16] Zaaijer HL, van Rixel T, van Exel-Oehlers P, et al. New anti-human immunodefieieney virus immunoblot assays resolve nonspecific western blot results. Transfusion,1997, 37:193-198.
[17] Imai M, Sudo K, Hayashi T, et al. Kinetic analysis of anti-HIV titer in early stage of HIV infeetion-a new testing strategy to differentiate early HIV infection individuals from false positive eases. Kansenshogaku Zasshi, 1999, 73:1183-1186.