中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (4): 197-199.

• 论著 • 上一篇    下一篇

产孢丝状真菌药敏试验检测暗色真菌对九种药物的敏感性

孙志坚, 李若瑜, 万喆, 王晓红, 王端礼   

  1. 北京大学第一医院皮肤科
  • 收稿日期:2003-04-26 出版日期:2004-04-15 发布日期:2004-04-15

Studies on Susceptibility of Nine Antifungal Agents Against Dematiaceous Fungi by NCCLS M38-P Method

SUN Zhi-jian, LI Ruo-yu, WAN Zhe, WANG Xiao-hong, WANG Duan-li   

  1. Department of Dermatology, The First Hospital of Peking University, Beijing 100034, China
  • Received:2003-04-26 Online:2004-04-15 Published:2004-04-15

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摘要： 目的 检测暗色真菌对9种常用抗真菌药物的敏感性,评价美国国家实验室标准委员会(NCCLS)推荐的产孢丝状真菌药敏试验方案(M38-P)应用于暗色真菌药敏试验的可行性.方法 参照M38-P方案,受试菌株共45株,包括卡氏枝孢14株,裴氏着色霉8株,紧密着色霉3株,疣状瓶霉16株,离蠕孢2株,德氏霉1株及弯孢1株.所用药物为氟康唑、氟胞嘧啶、伊曲康唑、咪康唑、益康唑、克霉唑、制霉菌素、两性霉素B和特比萘芬.菌悬液终浓度为(0.4~5)×106cfu/mL,孵育温度35℃,培养时间5~7d.结果 45株暗色真菌对伊曲康唑和特比萘芬的敏感性较高,部分菌株对氟康唑、氟胞嘧啶、伊曲康唑、两性霉素B存在耐药现象.结论 M38-P方案可用于暗色真菌的药敏试验研究,耐药菌株的发现提示在今后的治疗中药敏测试是必要的.

关键词: 有丝分裂孢子真菌, 抗生素类,抗真菌, 微生物敏感性试验

Abstract: Objectives To evaluate the application of M38-P method recommend by NCCLS to dematiaceous fungi, and detect the susceptibility of itraconazole, fluconazole, miconazole, econazole, flucytosine, clotrimazole, nystatin, amphotericin B and terbinafine against dematiaceous fungi. Methods Using M38-P method forty-five strains of dematiaceous fungi were studied, which included 2 strains of Bipolaris spp., 1 strain of Drechslera spp., 1 strain of Curvularia spp.,14 strains of C. carrionii, 8 strains of F. pedrosoi, 3 strains of F. compacta and 16 strains of P. verrucosa. The media containing the fungi in the concentrations of (0.4~5)×106 CFU/mL were incubated at 35℃ for 5~7 days. Results The tested dematiaceous fungi were highly susceptible to itraconazole and terbinafine. Some of them were resistant to itraconzole, fluconazole, flucytosine and amphotericin B. Conclusions The data suggest that M38-P standards could be applied as the susceptibility testing of dematiaceous fungi, and it is necessary to monitor the resistant strains in antifungal treatment.

Key words: Mitosporic fungi, Antibiotics, antifungal, Microbial sensitivity tests