中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 88-90.

• 论著 • 上一篇    下一篇

燃煤型砷中毒皮损中2个DNA微卫星位点的变化

袁伟1, 张信江1, 瓦庆彪1, 郑庭铭1, 肖和1, 杜辉2, 周运书2   

  1. 1. 遵义医学院附属医院皮肤性病科 贵州 563003;
    2. 贵州省黔西南州疾病控制中心 贵州 563003
  • 收稿日期:2003-02-20 出版日期:2004-02-15 发布日期:2004-02-15
  • 通讯作者: 张信江,E-mail:xinjiangzhang@sohu.com E-mail:xinjiangzhang@sohu.com
  • 基金资助:
    中华医学会皮肤科分会基金(200130);贵州省省长资金(C-197)

Detection of Microsatellite Instability in Lesions from Patients with Coal Arsenic Poisoning

YUAN Wei1, ZHANG Xin-jiang1, WA Qing-biao1, ZHENG Ting-ming1, XIAO He1, DU Hui2, ZHOU Yun-shu2   

  1. Department of Dermatology, Affiliated Hospital, Zunyi Medical College, Zunyi 563003, China
  • Received:2003-02-20 Online:2004-02-15 Published:2004-02-15

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摘要： 目的 探讨微卫星不稳定性及杂合性丢失在燃煤型砷中毒患者皮肤病变发展中的作用。方法 依据皮肤病理组织学检查结果对燃煤型砷中毒患者进行分组,选取TP53.PCR15、D9S3192个微卫星多态性标记,采用PCR扩增-变性聚丙烯酰胺凝胶电泳-银染法检测其微卫星不稳定性情况。结果 34份燃煤型砷中毒患者皮损组织微卫星不稳定性的发生率为32.4%(11/34),杂合性丢失的发生率为14.7%(5/34),微卫星不稳定性与临床分度、病理分型之间统计学分析差异无显著性(P>0.05)。结论 微卫星不稳定性和杂合性丢失可能在砷中毒皮损癌变的发生发展中起一定的作用。

关键词: 砷中毒, DNA,卫星, 三核苷酸重复扩增, 杂合子丢失

Abstract: Objective In order to elucidate the significance of both microsatellite instability(MSI) and loss of heterozygosity (LOH) in the pathogenesis of arsenic-induced skin lesions, especially skin carcinoma, the presence of MIS and LOH at two loci [chromosome 9p21 (D9S319, p16) and 17p13.1 (TP53.PCR15, p53)] was evaluated. Methods Using polymerase chain reaction(PCR)-denatured polyacrylamide gel electrophoresis-silver staining, MSI and LOH were detected in a total of 34 samples of arsenic-induced skin lesions, and the results were compared with the clinicopathological parameters. Results MSI was found in 32.4% (11/34), and LOH was found in 14.7%(5/34) of 34 samples of arsenic-induced skin lesions. There were no significant differences between the presence of MSI and clinical severity or pathological grades (P>0.05). Conclusions MSI and LOH may play a certain role in the carcinogenesis and progression of arsenic-induced skin lesions.

Key words: Arsenic poisoning, DNA, satellite, Trinucleotide repeat expansion, Loss of heterozy-gosity