中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 86-87.

• 论著 • 上一篇    下一篇

端粒酶在鳞状细胞癌和Bowen病组织中的表达

梁虹, 周心房, 方春红, 曹富英   

  1. 武汉大学人民医院皮肤科 武汉 430060
  • 收稿日期:2003-02-17 出版日期:2004-02-15 发布日期:2004-02-15

Expression of Telomerase in Cutaneous Squamous Cell Carcinoma and Bowen’s Disease

LIANG Hong, ZHOU Xin-fang, FANG Chun-hong, CAO Fu-ying   

  1. Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Received:2003-02-17 Online:2004-02-15 Published:2004-02-15

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摘要：

目的 探讨端粒酶在皮肤鳞状细胞癌(SCC)及Bowen病(BD)病变组织中的表达及其临床意义。方法 应用原位杂交方法检测30例皮肤SCC和30例BD病变组织中端粒酶基因hTR和hTERTmRNA的表达。结果 在BD组织中hTR和hTERTmRNA呈弱表达,阳性率为23.33%和16.67%。在SCC中,hTR和hTERTmRNA的阳性率分别为86.67%和93.33%。其中Ⅰ、Ⅱ级SCC阳性率分别为82.35%和88.24%,无强阳性;Ⅲ、Ⅳ级SCC阳性率分别为92.31%和100%、强阳性表达率分别为69.23%和76.92%。SCC和BD两者比较差异有显著性(P<0.05)。SCCⅠ、Ⅱ级与Ⅲ、Ⅳ级比较差异有显著性(P<0.05)。结论 端粒酶hTR和hTERTmRNA的表达增多是预测皮肤SCC的重要因素。

关键词: 端粒末端转移酶, 鳞状细胞癌, Bowen病

Abstract:

Objective To investigate the clinical significance of human telomerase RNA component (hTR) and human telomerase reverse transcriptase(hTERT) mRNA expression in cutaneous squamous cell carcinoma(SCC) and Bowen's disease(BD). Methods Expressions of hTR and hTERT mRNA were detected by in situ hybridization technique in 30 cases of cutaneous SCC and 30 cases of BD. Results Weak expressions of hTR and hTERT mRNA were observed in BD, with positivity rates of 23.33% and 16.67%, respectively. Expressions of hTR and hTERT mRNA were found in 86.67% and 93.33%, respectively, in cutaneous SCC. hTR and hTERT mRNA were positive in 82.35% and 88.24%, respectively, of stage I and II of cutaneous SCC, without strong positive signals in those patients. hTR and hTERT mRNA were positive in 92.31% and 100%, respectively, of stage III and IV of cutaneous SCC, with strong positivity rates of 69.23% and 76.92% in those patients. Expressions of hTR and hTERT mRNA in skin tumors were gradually increased which positively linked with phenotypic progression and degree of dysplasia. Conclusion The strong expression of hTR and hTERT mRNA in the skin tumor suggest the presence of cutaneous squamous cell carcinoma.

Key words: Telomerase, Carcinoma, squamous cell, Bowen’s disease