中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 80-82.

• 论著 • 上一篇    下一篇

皮肤结节性血管炎患者Th1/Th2型细胞因子表达的研究

郭在培1, 刘宏杰1, 刘卫平2, 王琳1, 李俸媛2, 杜晓萍1   

  1. 1. 四川大学华西医院皮肤性病科 成都 610041;
    2. 四川大学华西医院病理科 成都 610041
  • 收稿日期:2003-02-10 出版日期:2004-02-15 发布日期:2004-02-15

Expression of Th1/Th2 Cytokines in Patients with Cutaneous Nodular Vasculitis

GUO Zai-pei1, LIU Hong-jie1, LIU Wei-ping2, WANG Lin1, LI Feng-yuan2, DU Xiao-ping1   

  1. Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2003-02-10 Online:2004-02-15 Published:2004-02-15

PDF (PC)

172

赞

摘要： 目的 探讨CD3+、CD4+和CD8+T细胞和IL-2、IL-4、IL-6及sIL-2R与结节性血管炎患者发病、疾病活跃程度的关系。方法 采用流式细胞仪及ELISA法检测外周血CD3+、CD4+、CD8+T细胞亚群,血清IL-2、IL-6及sIL-2R水平,用链霉亲和素-过氧化物酶(SP)法检测皮损组织局部CD3+、CD4+、CD8+T细胞亚群浸润及IL-2、IL-4表达水平。结果 活动期结节性血管炎患者血清IL-2、IL-6及sIL-2R水平高于非活动期和正常人对照组;而外周血CD3+、CD4+、CD8+T细胞亚群与消退期及正常人比较差异无显著性。病变组织中CD3+、CD4+T细胞和IL-2染色阳性细胞高于正常人对照组;IL-4染色阳性细胞与正常人对照组差异无显著性。结论 IL-2为代表的Th1细胞因子可能参与结节性血管炎的发病机制,血清及组织浸润IL-2水平与疾病活跃相关。

关键词: 结节性, 细胞因子, 血管炎

Abstract: Objective To investigate the relationship between CD3+T, CD4+T, CD8+T cells, Th1/Th2 cytokines IL-2, IL-4, IL-6, sIL-2R and the pathogenesis as well as disease activity in patients with cutaneous nodular vasculitis (NV). Methods Cytokines IL-2, IL-6, sIL-2R and cell surface markers CD3+, CD4+ and CD8+ were detected by flow cytometry and ELISA with peripheral blood mononuclear cells from patients and controls. Expressions of IL-2, IL-4 and CD3+, CD4+, CD8+ were determined by immunohistochemistry in patients' lesions in situ. Results Serum levels of IL-2, IL-6 and sIL-2R were significantly increased in patients with active NV compared with those in inactive stage(P<0.01) and in controls(P<0.01). There were no significant differences of the percentage of CD3+T, CD4+ and CD8+T in peripheral blood between patients with active NV and patients in stable stage, as well as controls. The numbers of CD3+T, CD4+T cells and IL-2-positive cells, except IL-4 positive cells, were significantly increased in biopsy tissues from NV patients compared with those from normal controls (P<0.01). Conclusions Th1 immune response, as indicated by the predominance of IL-2, may be involved in the pathogenesis of nodular vasculitis. It is suggested that IL-2 in both sera and tissue infiltration be associated with the disease activity.

Key words: Vasculitis, Nodular, Cytokine