中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 718-720.

• 论著 • 上一篇    下一篇

链格孢对ICR小鼠致病性的研究

王晓慧1, 张峰1, 李若瑜2, 涂平2   

  1. 1. 哈尔滨医科大学第一临床医院皮肤科 150001;
    2. 北京大学第一医院皮肤科
  • 收稿日期:2003-12-16 出版日期:2004-12-15 发布日期:2004-12-15
  • 基金资助:
    中华医学会皮肤科学会诺华科研基金资助

Pathogenicity of Alternaria alternata in ICR Mice

WANG Xiao-hui1, ZHANG Feng1, LI Ruo-yu2, TU Ping2   

  1. Department of Dermatology, First Hospital, Harbin Medical University, Harbin 150001, China
  • Received:2003-12-16 Online:2004-12-15 Published:2004-12-15

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摘要： 目的 探讨链格孢对ICR小鼠的致病性.方法 将链格孢的菌悬液分别接种于正常和免疫抑制ICR小鼠的睾丸和足垫,于接种后的第5、7、10、14、21、28、35和42天处死动物,取接种部位的组织和各脏器进行组织病理学检查和逆培养.结果 正常组和免疫抑制组小鼠的接种部位均出现脓肿,免疫抑制组较正常组组织损害程度重,且持续时间长;足垫接种小鼠在正常组脓肿14d自行消退,在免疫抑制组28～35d消退.正常组和免疫抑制组小鼠的接种部位组织逆培养均阳性,组织病理观察见脓肿、粗大菌丝、肉芽肿.实验小鼠均未出现系统播散.结论 链格孢对ICR小鼠是致病性很弱的机会致病菌.

关键词: 链格孢属, 真菌病, 动物替代试验

Abstract: Objective To investigate the pathogenicity of Alternaria alternata in ICR mice.Methods The cell suspension of Alternaria alternata was inoculated into testes and footpads of normal and immunocompromised ICR mice.Histopathologic examination and fungal culture were performed on 5th d, 7th d, 10 th d, 14th d, 21st d, 28th d, 35th d and 42nd d respectively after inoculation.Results The abscesses were developed at the inoculated sites in all normal and immunocompromised mice.The lesions in immunocompromised mice were more severe and persisted longer than those in normal mice.The abscesses in footpads mostly disappeared within 14 days in normal mice but in 28 to 35 days in immunocompro mised mice.Alternaria alternata was isolated from the inoculated tissues in both groups.The histopathologic examination showed abscess and granulomas in which thick hyphae were observed.The necropsy revealed no disseminated infection of Alternaria alternate in all the test mice.Conclusion Alternaria alternata is a kind of opportunistic fungus with low virulence.

Key words: Alternaria, Mycoses, Animal testing alternatives