中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 703-705.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者血清肝细胞生长因子及基质金属蛋白酶9的研究

刘旸1, 郑敏2, 殷文浩2, 鲍彰2   

  1. 1. 浙江大学医学院附属第二医院风湿病学教研室 杭州 310009;
    2. 浙江大学医学院附属第二医院皮肤科
  • 收稿日期:2003-12-29 出版日期:2004-12-15 发布日期:2004-12-15
  • 基金资助:
    浙江省自然科学基金资助课题(399126)

Detection of Serum HGF and MMP-9 and Its Clinical Significance in Pat ients with Systemic Lupus Erythematosus

LIU Yang1, ZHENG Min2, YIN Wen-hao2, BAO Zhang2   

  1. Department of Rheumatology, the Second Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310009, China
  • Received:2003-12-29 Online:2004-12-15 Published:2004-12-15

PDF (PC)

93

赞

摘要： 目的 探讨血清肝细胞生长因子(HGF)及基质金属蛋白酶9(MMP-9)的水平与系统性红斑狼疮(SLE)疾病活动程度的关系和意义,并对相关机理进行探讨.方法 ①双抗体夹心法检测血清HGF及MMP-9水平;②绘制ECV304细胞株生长曲线;③明确重组人肝细胞生长因子(rhHGF)的作用浓度;④流式细胞仪检测MMP-9水平.结果 患者血清HGF明显升高(P<0.001),MMP-9明显降低(P<0.001);经糖皮质激素治疗后HGF浓度下降(P<0.05),MMP-9浓度升高(P<0.05);活动期HGF高于非活动期(P<0.05),MMP-9低于非活动期(P<0.05);肾损组HGF高于非肾损组(P<0.001),MMP-9则低于非肾损组(P<0.05);关节炎组HGF高于非关节炎组(P<0.01),MMP-9水平两组之间差异无显著性(P>0.05);以HGF水平作为SLE的诊断指标,则ROC曲线下面积为0.707,灵敏度为66.7%;以MMP-9水平作为SLE的诊断指标,则ROC曲线下面积为0.984,灵敏度为97.2%;同时检测HGF及MMP-9水平,灵敏度为66.7%(24/36);rhHGF的作用浓度为8ng/mL;正常ECV304细胞株MMP-9的表达量为39.74%,rhHGF刺激后MMP-9的表达量为40.32%.结论 HGF及MMP-9可能参与SLE的发病机制,其水平可作为反映疾病活动程度、肾脏损害以及疾病进展与改善的指标,且血清MMP-9水平诊断准确性更高;rhHGF可促进ECV304细胞株MMP-9的表达.

关键词: 红斑狼疮,系统性, 肝细胞生长因子, 明胶酶B

Abstract: Objectives To explore the relationship between serum levels of hepatocyte growth factor (HGF) and matrix metalloproteinase-9 (MMP-9) and the disease activity of systemic lupus erythematosus (SLE), and study the mechanisms of these two factors in the pathogenesis of SLE.Methods The serum levels of HGF and MMP-9 were measured by ELISA.The growth curve of normal ECV304 cell line was obtained, and the action concentration of recombinant human hepatocyte growth factor (rhHGF) was determined.MMP-9 expression level in cells was detected by flow cytometric analysis.Results The serum level of HGF increased significantly in SLE patients as compared with that in healthy controls (P<0.001), but the serum level of MMP-9 decreased significantly (P<0.001).The serum level of HGF decreased after treatment (P<0.05), but MMP-9 increased (P<0.05).The serum level of HGF was higher in patients with active disease than that in patients with inactive disease (P<0.05), but MMP-9 was lower (P<0.05).The serum level of HGF increased in patients with renal damage as compared with those without renal damage (P<0.001), but the serum level of MMP-9 decreased (P<0.05).The serum level of HGF was higher in patients with arthritis than those without (P<0.01), but MMP-9 serum level had no significant difference in these two groups (P>0.05).The area of ROC curve was 0.707 and the sensitivity was 66.7% when using the serum level of HGF as diagnostic standard.The area under ROC curve was 0.984 and the sensitivity was 97.2% when using the serum level of MMP-9 as diagnostic standard.The sensitivity was 66.7% (24/36) when two markers (HGF and MMP-9) were examined simultaneously.Additionally, the action concentrati on of rhHGF was 8 ng/mL, and the expression level of MMP-9 was 39.74% in normal ECV304 cells and increased to 40.32% after rhHGF stimulation.Conclusions It is suggested that HGF and MMP-9 may be involved in the pathogenesis of SLE, and s erum levels of HGF and MMP-9 might be used as markers for monitoring the disease activity, renal damage, disease progression and improvement in SLE.The sensitivity might be higher when serum level of MMP-9 is used as diagnostic standard, and rhHGF can enhance MMP-9 expression in ECV304 cell line.

Key words: Lupus erythematosus,systemic, Hepatocyte growth factor, Gelatinase B