中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 692-694.

• 论著 • 上一篇    下一篇

衡阳和江门地区梅毒螺旋体基因分型的初步研究

曾铁兵1, 吴移谋1, 黄澍杰2, 吴志周2   

  1. 1. 南华大学医学院微生物学教研室 衡阳 421001;
    2. 广东江门市皮肤病医院
  • 收稿日期:2004-03-02 出版日期:2004-12-15 发布日期:2004-12-15
  • 基金资助:
    湖南省教育厅资助重点课题(02A046);湖南省卫生厅资助课题(Y02-067)

Preliminary Study on Molecular Subtyping of Treponema pallidum in Hen gyang and Jiangmen Regions

ZENG Tie-bing1, WU Yi-mou1, HUANG Shu-jie2, WU Zhi-zhou2   

  1. Department of Microbiology, Nanhua University, Hengyang 421001, China
  • Received:2004-03-02 Online:2004-12-15 Published:2004-12-15

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摘要： 目的 了解湖南衡阳和广东江门地区梅毒螺旋体(TP)基因亚型的分布.方法 收集衡阳和江门地区疑似硬下疳病例的标本85份,经TP polA PCR筛选后,阳性者用PCR扩增各标本中TP的arp和tpr基因,酶切tpr基因扩增产物,分析各TP菌株arp基因长度和tpr基因的限制性片段长度多态性(RFLP),进行分型.结果 TP polA PCR阳性者69例,可用于分型者57例,分为10个亚型,其中14d亚型为26例,其他亚型包括10d(1),12a(3),12g(2),13d(6),14a(5),14b(2),14f(6),15d(5),16d(1);衡阳和江门地区TP基因亚型分布大致相同.结论 衡阳和江门地区存在TP的多亚型,都以14d亚型为主(占45.6%),无明显地区性差异.

关键词: 密螺旋体,苍白, 分子亚型

Abstract: Objective To investigate the distribution of subtypes of T.pallidum (TP) in Hengyang and Jiangmen regions.Methods Eighty-five specimens taken from patients with suspected chancre collected in Hengyang and Jiangmen from February 2002 to January 2004 were screened by a PCR targeted TP polA gene, and then the arp gene and tpr gene were amplified from TP positive specimens.The PCR products of tpr gene were digested by restriction endonuclease MSe I.The sizes of the arp gene and the restriction fragment length polymorphism (RFLP) of the tpr gene were analyzed for subtyping.Results Of 69 TP-positive specimens, 57 could be subtyped, and 10 subtypes were found.Among them, 26 (45.6%) were subtype 14d, and other subtypes included 10d(1), 12a(3), 12g(2), 13d(6), 14a(5 ), 14b(2), 14f(6), 15d(5) and 16d(1).No significant difference of the distribution of TP subtypes between Hengyang and Jiangmen was found.Conclusion Multiple T.pallidum subtypes have been prevalent in Hengyang and Jiangmen, although the predominant subtype is 14d, there is no significant geographic heterogeneity between these two regions.

Key words: Treponema pallidum, Molecular subtype