中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 690-691.

• 论著 • 上一篇    下一篇

建立免疫调节剂体外诱导小鼠脾细胞生成Th1型细胞因子的反应模式

周小勇, 郑家润, 李新宇   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2003-12-18 出版日期:2004-12-15 发布日期:2004-12-15

Study on the In Vitro Model for Th1 Type Shifting

ZHOU Xiao-yong, ZHENG Jia-run, LI Xin-yu   

  1. Institute of Dermatology, Chine se Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2003-12-18 Online:2004-12-15 Published:2004-12-15

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摘要： 目的 建立小鼠脾细胞体外生成Th1型细胞因子的反应模式,以适应研究免疫治疗药物作用机制的要求.方法 ELISA检测单核/巨噬细胞系统活化剂脂多糖(LPS)或T细胞丝裂原伴刀豆球蛋白A(ConA)在不同剂量或两种诱导剂不同剂量组合的条件下,诱导BALB/c小鼠脾细胞分泌的白介素12(IL-12)、干扰素γ(IFN-γ)和白介素4(IL-4)的产量.结果 LPS能诱导BALB/c小鼠脾细胞分泌IL-12.ConA诱导BALB/c小鼠脾细胞分泌IFN-γ的最低质量浓度是0.25mg/mL.不同浓度LPS与0.25mg/mLConA联合诱导BALB/c小鼠脾细胞分泌IFN-γ时,LPS表现出协同效应,IL-12与IFN-γ呈正相关,IL-4与IFN-γ呈负相关.结论 LPS和ConA体外联合可诱导鼠脾细胞向Th1型细胞反应方向发展.

关键词: 白细胞介素12, 白细胞介素4, 干扰素Ⅱ型, 脂多糖类, 伴刀豆球蛋白A

Abstract: Objectives To establish an experimental model for Th1 type shifting and meet the requirements of studying on the mechanisms of some immunomodulators.Methods The levels of cytokines, IL-12, IFN-γ and IL-4, produced in vitro by spleen cells of the BALB/c mice were detected by using ELISA.Spleen cells of the BALB/c mice were incubated under the following conditions: with different concentrations of T cell mitogen ConA (1 mg/mL, 0.5 mg/mL, 0.25 mg/mL, 0.125 mg/mL), mononuclear phagocyte system activator LPS (50 mg/mL, 5 mg/mL, 0.5 mg/mL) or LPS (50 mg/mL, 5 mg/mL, 0.5 mg/mL) combined with 0.25 mg/mL ConA.Results LPS could induce the production of IL-12 from spleen cells.The lowest concentration that ConA could induce the measurable production of IFN-γ from spleen cells was 0.25 mg/mL.When different concentrations of LPS were combined with 0.25 mg/mL ConA, LPS could accelerate the production of IFN-γ and positively with that of IL-12.Conclusion LPS combined with ConA can induce the activation of spleen cells from mice towards Th1 type response.

Key words: Interleukin-12, Interleukin-4, Interferon type Ⅱ, Lipopolysa cchrides, Concanavalin A