阴道分离的念珠菌体外抗真菌药物敏感性分析

中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (11): 638-641.

阴道分离的念珠菌体外抗真菌药物敏感性分析

廉翠红, 崔凡, 沈永年, 陈伟, 刘维达

中国医学科学院、中国协和医科大学皮肤病研究所南京 210042

收稿日期:2003-11-01 出版日期:2004-11-15 发布日期:2004-11-15
通讯作者: 刘维达, E-mail:liumyco@hotmail.com E-mail:liumyco@hotmail.com
基金资助:
国家教委高校博士点基金课题资助(20010023019)

Analysis of In Vitro Antifungal Susceptibility of Vaginal Isolates of Candida spp.

LIAN Cui-hong, CUI Fan, SHEN Yong-nian, CHEN Wei, LIU Wei-da

Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China

Received:2003-11-01 Online:2004-11-15 Published:2004-11-15

摘要/Abstract

摘要： 目的探讨念珠菌在女性阴道黏膜所致的不同表现与其对抗真菌药物敏感性之间的关系。方法应用NCCLSM27-A方案推荐的微量液体稀释法,测定对来自外阴阴道念珠菌健康携带者、外阴阴道念珠菌病和复发性外阴阴道念珠菌病的念珠菌对抗真菌药物的敏感性。结果 8种抗真菌药对光滑念珠菌和克柔念珠菌的MIC值几乎均比白念珠菌高。来自复发性外阴阴道念珠菌病患者的菌株MIC值较高,而健康带菌者菌株的MIC值较低。耐药菌株主要来源于复发组,健康带菌者来源的白念珠菌菌株对伊曲康唑、氟康唑、酮康唑和制霉菌素未见有耐药菌株出现。结论外阴阴道念珠菌病治疗中应重视非白念珠菌的耐药性,长期或间断应用抗真菌药物治疗,可能诱导耐药株的产生。

关键词: 念珠菌病,外阴阴道, 念珠菌属, 抗真菌药, 微生物敏感性试验

Abstract: Objectives To analyse the antifungal susceptibility of Candida spp.isolated from the patients with recurrent vulvovaginal candidiasis (RVVC),vulvovaginal candidiasis (VVC) and asymptomatic carriers and to study the correlation between different Candida strains and antifungal susceptibility.Method According to the NCCLS-M27-A scheme,the antifungal susceptibility of Candida spp.isolated from the above different groups was measured.Results Almost all the MICs of C. glabrata and C. krusei to 8 antifungal agents were higher than those of C. albicans .The average MIC of C. albicans isolated from RVVC patients was higher than that from asymptomatic carriers.The resistant strains were mainly isolated from the RVVC group.No resistant strains against itraconazole,fluconazole,ketoconazole,econazole and nystatin was found in asymptomatic carriers.Conclusions These results indicate that more attention has to be paid to the low susceptibility of non-Candida albicans in the treatment of vulvovaginal candidiasis,and the resistant strains may result from long-term or irregular antifungal treatment.

Key words: Candidiasis,vulvovaginal, ^Candida, Antifungal agents, Microbial sensitivity tests

廉翠红, 崔凡, 沈永年, 陈伟, 刘维达. 阴道分离的念珠菌体外抗真菌药物敏感性分析[J]. 中华皮肤科杂志, 2004, 37(11): 638-641.

LIAN Cui-hong, CUI Fan, SHEN Yong-nian, CHEN Wei, LIU Wei-da. Analysis of In Vitro Antifungal Susceptibility of Vaginal Isolates of Candida spp.[J]. Chinese Journal of Dermatology, 2004, 37(11): 638-641.

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