中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (11): 632-634.

• 论著 • 上一篇    下一篇

大鼠皮肤角质层对荧光素钠脂质体透皮吸收的影响

曾抗1, 颉玉胜3, 李国锋2, 董平1, 朱晓亮1, 孙乐栋1, 后桂荣1   

  1. 1. 南方医科大学南方医院皮肤科 广州 510515;
    2. 南方医科大学南方医院药学部 广州 510515;
    3. 兰州空军医院皮肤科
  • 收稿日期:2003-11-03 出版日期:2004-11-15 发布日期:2004-11-15
  • 通讯作者: 颉玉胜
  • 基金资助:
    国家自然科学基金资助课题(39970680)

Influence of Stratum Corneum on the Percutaneous Absorption of Fluorescein Sodium Liposome in Rat Skin

ZENG Kang1, XIE Yu-sheng3, LI Guo-feng2, DONG Ping1, ZHU Xiao-liang1, SUN Le-dong1, HOU Gui-rong1   

  1. Department of Dermatology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China
  • Received:2003-11-03 Online:2004-11-15 Published:2004-11-15

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摘要： 目的 探讨大鼠皮肤角质层对荧光素钠脂质体透皮吸收的影响。方法 采用Scotch胶带剥脱皮肤角质层,利用荧光光度计、Franz扩散池和荧光显微镜研究皮肤角质层和活性层中荧光素钠的含量;观察皮肤剥脱角质层后荧光素钠脂质体透皮吸收能力的变化和荧光素钠在皮肤中的分布。结果 应用脂质体后皮肤各层荧光素钠的分布量均较相应溶液和凝胶作用后增加(P<0.01);荧光素钠在皮肤各层的分布比例发生了变化;剥脱角质层后脂质体荧光素钠混悬液和荧光素钠溶液各时间点累积透皮浓度相比较,差异无显著性(P>0.05);脂质体荧光素钠凝胶和荧光素钠凝胶相比较,各时间段累积透皮浓度差异无显著性(P>0.05)。结论 脂质体能增加皮肤各层的荧光素钠含量,并能够改变皮肤角质层和去角质层皮肤之间的荧光素钠比例。剥脱角质层后脂质体制剂透皮量显著增加,与相应的普通制剂相比差异无显著性;剥脱角质层后脂质体制剂的毛囊扩散途径不发生改变。

关键词: 表皮, 荧光素, 脂质体, 皮肤吸收, 大鼠,Sprague-Dawley

Abstract: Objective To study the effects of stratum corneum on the percutaneous absorption of fluorescein sodium (NaFl) liposome in rat skin.Methods Stratum corneum was stripped off by Scotch adhesive tape,and the NaFl content in the stratum corneum and the skin with no stratum corneum were detected by spectrofluorimetry at regular intervals.The dynamic permeated amount of different preparations of NaFl in the skin at different intervals in Franz diffuse cell was determined and the distribution of NaFl at 4 h after stripping off the stratum corneum was observed by fluorescence microscope.Results The concentrations of NaFl in the stratum corneum and the skin with no stratum corneum after applying liposome NaFl preparation were significantly higher than those of NaFl solution and gel preparations (P<0.01).Also,the ratio of NaFl distribution in different skin layers changed significantly.However,compared with the control groups,the accumulated percutaneous concentrations of different liposome formulations in receiver cell of Franz diffuse cell had no significant difference (P>0.05).Conclusions It is suggested that liposome can increase and change the penetration of NaFl into the stratum corneum and the skin with no stratum corneum in vitro.Hair follicular structure may not play an important role in the drug diffusion when stratum corneum is removed.

Key words: Epidermis, Fluorescein, Liposomes, Skin absorption, Rats,Sprague-Dawley