中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (10): 572-574.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞Toll样受体4及2mRNA表达的研究

沈小雁1, 薛峰1, 陈晓鸿2, 李霞1, 徐涵1, 郑捷1   

  1. 1. 上海第二医科大学附属瑞金医院皮肤科、风湿科 200025;
    2. 上海东方医院检验科 200025
  • 收稿日期:2003-10-02 出版日期:2004-10-15 发布日期:2004-10-15
  • 基金资助:
    上海市卫生局2003年科研资助项目(024082)

Expression of TLR4 and TLR2 mRNA in the Peripheral Blood Mononuclear Cells of the Patients with Systemic Lupus Erythematosus

SHEN Xiao-yan1, XUE Feng1, CHEN Xiao-hong2, LI Xia1, XU Han1, ZHENG Jie1   

  1. Department of Dermatology & Rheumatology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
  • Received:2003-10-02 Online:2004-10-15 Published:2004-10-15

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摘要： 目的 研究系统性红斑狼疮(SLE)患者与正常人外周血单一核细胞Toll样受体4(TLR4)mRNA、TLR2mRNA的表达情况。方法 运用Taqman实时定量PCR方法检测活动期SLE患者28例、稳定期SLE患者13例外周血单一核细胞TLR4、TLR2的mRNA表达水平,并与正常人作对照。结果 活动期SLE患者外周血单一核细胞TLR4mRNA较正常人和稳定期SLE患者表达水平显著升高(P<0.01);其中复发的、经糖皮质激素治疗的活动期SLE患者较未经治疗的初发患者TLR4mRNA升高更明显(P<0.05)。SLE活动期和稳定期患者TLR2mRNA较正常人对照显著降低(P<0.01)。结论 活动期SLE患者TLR4mRNA表达水平升高,尤其是使用过糖皮质激素的患者TLR4升高更为显著,其原因可能与感染作为诱发SLE活动因素之一有关。

关键词: 红斑狼疮, 系统性, Toll样受体

Abstract: Objective To investigate the mRNA expression of TLR4 mRNA and TLR2 in the peripheral blood mononuclear cells(PBMC)from the patients with systemic lupus erythematosus (SLE). Methods The mRNA of TLR4 and TLR2 were detected in the patients with SLE (n=41) and the normal healthy control (n=24) with the method of Taqman Real-time PCR. Results The mRNA expression of TLR4 was significantly higher in active SLE patients than that in inactive SLE patients and the normal healthy control (P<0.01), and was especially higher in the relapsed patients and active SLE received the treatment of glucocorticoid (GC) than that in the patients who were never treated with GC (P<0.05). But comparing with the normal healthy controls, the mRNA expression of TLR2 was significant lower either in the patients with active or inactive SLE (P<0.01). Conclusion The higher expression of TLR4 mRNA in active SLE patients indicates that infection may be one of the precipitating factors of SLE or SLE relapse.

Key words: Lupus erythematosus, systemic, Toll-like receptors